华蟹甲根茎中酚类成分研究

Study of phenolic compounds from rhizome of Sinacalia tangutica

目的研究华蟹甲Sinacalia tangutica根茎的化学成分。方法采用硅胶、聚酰胺、Sephadex LH-20、反相ODS等柱色谱方法对化合物进行分离,运用核磁共振波谱等方法鉴定化合物的结构。结果从华蟹甲根茎的乙醇提取物中分离得到14个酚类化合物:7-羟基-8-甲氧基香豆素(1)、7-羟基香豆素(2)、丁香酸(3)、4-羟基-3,5-二甲氧基苯甲醛(4)、3,4-二羟基苯甲醛(5)、对羟基苯甲醛(6)、咖啡酸(7)、对羟基桂皮酸(8)、阿魏酸(9)、4-咖啡酰基奎宁酸甲酯(10)、5-咖啡酰基奎宁酸甲酯(11)、5-咖啡酰基奎宁酸乙酯(12)、4,5-二咖啡酰基奎宁酸甲酯(13)、3,5-二咖啡酰基奎宁酸甲酯(14)。结论化合物3~5、7~14为首次从该属植物中分离得到。

Objective To study the chemical constituents of the rhizome of Sinacalia tangutica. Methods The constituents were isolated and purified by silica gel, polyamide column chromatography, Sephadex LH-20, and ODS column chromatography. Their structures were elucidated by NMR spectra comparison with reference data. Results Fourteen phenolic compounds were isolated from the rhizome of S. tangutica, which were identified as hydrangetin （1）, 7-hydroxy-coumarin （2）, syringic acid （3）, 4-hydroxy-3,5-dimethoxybenzaldehyde （4）, 3,4-dihydroxybenzaldehyde （5）, 4-hydroxybenzaldehyde （6）, caffeic acid （7）, p-hydroxycinnamic acid （8）, ferulic acid （9）, methyl 4-O-caffeoyl quinate （10）, methyl 5-O-caffeoyl quinate （11）, ethyl 5-O-caffeoyl quinate （12）, methyl 4,5-di-O-caffeoyl quinate （13）, and methyl 3,5-di-O-caffeoyl quinate （14）. Conclusion Compounds 3—5, 7—14 are isolated from the genus of Sinacalia for the first time.