印记基因H19和SNRPN CpG位点甲基化状态与精液参数的关联研究

Association between methylation status of CpG sites of imprinted gene H19 and SNRPN and semen parameters

目的:分析印记基因H19和SNRPN印记控制区的各Cp G位点甲基化状态与男性精液参数的关联。方法:选取2014年4至9月于空军总医院男科就诊的符合纳入排除标准的205例男性为研究对象,采用焦磷酸测序法定量分析H19和SNRPN基因的各Cp G位点的甲基化状态,采用典型相关分析印记基因H19和SNRPN的各Cp G位点的甲基化水平与精液参数两组变量的整体相关性。结果:H19基因的Cp G2和Cp G3甲基化水平与精子密度和精子活动力的相关性具统计学意义;SNRPN基因的Cp G5、Cp G6、Cp G7甲基化水平与精子正常形态率的相关性具统计学意义。结论:印记基因H19和SNRPN印记控制区特定Cp G位点甲基化状态与精液参数有关联。

Objectives： To investigate the association between methylation status of imprinted gene H19 and SNRPN Cp G sites and semen parameters. Methods： A total of 205 men who visited the Andrology Clinic of Integrated Chinese and Western Medicine of the Air Force General Hospital,from April to September in 2016,and met the inclusion and exclusion criteria,were included in this study. The methylation status of Cp G sites on imprinted gene H19-ICR and SNRPN-ICR was measured quantitatively by pyrosequencing. Results： The typical correlation analysis found that the methylation status of Cp G2 and Cp G3 on imprinted gene H19 was significantly correlated with sperm density and sperm motility,while the methylation status of Cp G5,Cp G6 and Cp G7 on imprinted gene SNRPNwas significantly correlated with percentage of morphologically normal sperm. Conclusion： The methylation status of specific Cp G sites on imprinted gene H19 and SNRPNICR are associated with semen parameters.