寻常性银屑病皮肤屏障功能及丝聚蛋白表达水平的检测及意义

Measurement and Significance of Skin Barrier and Filaggrin Level in Psoriasis Vulgaris

目的通过两种不同的方法对咪喹莫特(imiquimod,IMQ)诱发银屑病样小鼠模型进行皮肤屏障功能检测,并通过寻常性银屑病患者皮损中皮肤屏障功能生物学指标的变化证实皮肤屏障功能参与银屑病的发病过程,从而对早期银屑病的临床治疗提供一定的理论依据。方法通过HE及免疫荧光染色证实IMQ可诱发银屑病样炎症,通过伊文思蓝(evans blue)染料渗出实验和异硫氰酸荧光素(FITC)结合血清白蛋白渗透性实验评估小鼠皮肤屏障功能,应用免疫组化及Real-time PCR对银屑病患者皮损中丝聚蛋白的表达水平进行检测。结果在咪喹莫特诱发银屑病样小鼠模型中,伊文思蓝染料渗出实验及异硫氰酸荧光素(FITC)结合血清白蛋白渗透性实验结果显示,银屑病模型组皮肤由内向外的渗透性及由外向内的渗透性较正常对照组均增加,差异有统计学意义(P<0.05),说明银屑病模型组小鼠的皮肤屏障功能较正常对照组减弱;寻常性银屑病患者皮损中丝聚蛋白表达水平及m RNA表达水平均较正常人降低(P<0.05),说明皮肤屏障功能亦有受损。结论皮肤屏障破坏存在于寻常性银屑病的发病过程中,恢复银屑病患者的皮肤屏障功能应当作为银屑病治疗的重要内容之一,在银屑病治疗中早期就应当重视应用止痒药物及保湿剂,从而加快皮肤屏障的修复。

Objective To investigate the barrier function in psoriasis-like animal model induced by IMQ and patients with psoriasis vulgaris, so as to provide an academic evidence for skin barrier involvement and psoriasis therapy. Methods Mouse was topically applied with IMQ, and the skin inflammation was analyzed by using H/E staining and immunoflourescense. The barrier function of mouse was analyzed by Evans Blue extravasation assay and FITC-conjuncted albumin assay. The expression level of filaggrin in psoriasis vulgaris was detected by immunohistochemistry and Real-time PCR analysis.Results In mouse model study, the results of Evans Blue extravasation assay and FITC-conjuncted albumin assay were much higher than that in control groups（P〈 0.05）, which indicated that the barrier function was defected in IMQ induced psoriasis mouse model. In addition, the expression of filaggrin in patients with psoriasis vulgaris decreased compared to the healthy people（P〈0.05）by using immunohistochemistry and RT-PCR which showed a defective barrier in psoriasis patients as well. Conclusion A psoriasis mouse model can be induced by IMQ in our study; skin barrier is involved in the development of psoriasis which provides an academic evidence of recovering the barrier as one of the main therapy of psoriasis onset by using emollient and sedating pruritus.