弥漫大B细胞淋巴瘤中SEL1L、BCL-2的表达及意义

Expression of SEL1L and BCL-2 in diffuse large B cell lymphoma

目的探讨SEL1L蛋白、BCL-2蛋白在弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织和细胞株SUDHL-4、LY-10中的表达及意义。方法采用免疫组化法检测123例DLBCL组织中SEL1L、BCL-2蛋白的表达及60例反应性淋巴组织增生(reactive lymphoid hyperplasia,RLH)组织中SEL1L蛋白的表达。应用免疫细胞化学及Western blot法检测SEL1L蛋白在DLBCL细胞株SUDHL-4及LY-10中的表达。结果 SEL1L蛋白在DLBCL中的高表达率明显高于RLH(69.9%vs 25.0%,P<0.05),其表达与各临床病理特征均无关。BCL-2蛋白在DLBCL中的阳性率为83.7%,与肿瘤原发位置、免疫表型及Ann Arbor分期有关(P<0.05);SEL1L蛋白与BCL-2蛋白在DLBCL中的表达呈正相关(r=0.227,P<0.05)。SEL1L蛋白在SUDHL-4及LY-10中均呈阳性。结论 SEL1L与BCL-2在DLBCL中均呈高表达,提示SEL1L在DLBCL的发生、发展中可能起重要作用,并可能与BCL-2表达产生协同作用。

Purpose To investigate the expression and the clinical significances of SEL1L and BCL-2 in 123 cases of diffuse large B cell lymphoma （DLBCL） and cell line SUDHL-4, LY-10. Methods Immunohistochemistry staining for SEL1L was performed in 123 DLBCL and 60 reactive lymphoid hyperplasia （RLH）, and also BCL-2 protein in 123 DLBCL. Immunocytochemistry staining and Western blot analysis for SEL1L protein were used in SUDHL4 and LY-IO. Results The high expression rate of SEL1L was 69. 9% in 123 DLBCL, which was significantly higher than that in 60 RLH （25.0%）. The expression of SELl L protein in DLBCL was not related to clinic pathological parameters. The positive rate of BCL-2 was 83.7% in 123 DLBCL. The expression of BCL-2 protein was correlated with immunophenotyping, primary location, and Ann Arbor stage. The expression of SELl L protein was positively correlated with that of BCL-2 protein in DLBCL （ r = 0. 227, P 〈 0. 05 ）. SELIL protein was also detected in SUDHL-4 and LY-10 cell lines. Conclusion The SEL1L protein may play an important role in the carcinogenesis of DLBCL, and may be associated with BCL-2.