甲状腺良恶性肿瘤中IMP3的表达及意义

Expression of IMP3 in benign and malignant thyroid tumor

目的探讨IMP3在甲状腺良恶性肿瘤组织中的表达及意义。方法运用荧光定量PCR与免疫组化法检测IMP3 mRNA及蛋白在71例不同病理类型甲状腺组织中的表达,分析其在甲状腺良恶性肿瘤鉴别诊断中的意义。结果与正常甲状腺组织相比,不同甲状腺病变组织中IMP3 mRNA表达水平均显著上调,由高到低依次为甲状腺滤泡亚型乳头状癌(follicular variant of papillary thyroid carcinoma,FVPTC)(10.13倍)、甲状腺滤泡癌(follicular thyroid carcinoma,FTC)(8.81倍)、甲状腺非滤泡型乳头状癌(papillary thyroid carcinoma,PTC)(8.52倍)、结节性甲状腺肿(nodular goiter,NG)(2.46倍)、甲状腺滤泡性腺瘤(follicular adenoma,FA)(1.49倍);IMP3蛋白阳性率也显著升高,由高到低依次为FTC(100%)、PTC(96.77%)、FVPTC(90%)、FA(20%)、NG(0);IMP3蛋白表达与mRNA表达呈正相关(P<0.01)。甲状腺恶性肿瘤组的IMP3 mRNA表达水平(8.82倍)明显高于良性病变组(1.94倍)(P<0.01);恶性滤泡性病变组的IMP3表达水平(9.36倍)也显著高于良性滤泡性病变组(1.49倍)(P<0.01)。结论 IMP3是甲状腺良恶性肿瘤诊断的有效分子标志物,也是甲状腺良恶性滤泡性病变的鉴别诊断指标。

Purpose To investigate the expression of insulin-like growth factor II mRNA-binding protein 3 （IMP3） and its differential diagnostic significance in benign and malignant thyroid tumor. Methods The fluorescent quantitative PCR and immunohistochemical staining were used to detect the IMP3 expression in 71 cases of thyroid tissue of different pathological types. The differential diagnostic significance of IMP3 expression in benign and malignant thyroid tumor was analyzed. Results Compared with normal thyroid tissue, thyroid tumors including follicular variant of papillary thyroid carcinoma （FVPTC）, follicular thyroid carcinoma （FTC） , papillary thyroid carcinoma （ PTC）, nodular goiter （ NG ） , and follicular adenoma （ FA ） had significantly higher IMP3 mRNA expression levels with 10. 13, 8.81, 8.52, 2. 46, and 1.49 holds, respectively. The positive expression rate of IMP3 protein in thyroid tumors were significantly higher, with the positive rate from high to low was FTC （100%）, PTC （96.77%）, FVI~＇FC （90%）, FA （20%）, and NG （0）. The expression level of IMP3 protein was positively correlated with the expression of mlINA （ P 〈 0. 01）. The IMP3 expression level of malignant thyroid tmnor （8.82 holds） was significantly higher than that o1＇ benign thyroid tumor （ 1.94 holds） （P 〈0. 01 ）. The IMP3 expression level of malignant thyroid follicular lesions （ 9.36 hohts ） was higher than that of benign thyroid follicular lesions （ 1.49 holds ） （ P 〈 0. 01）. Conclusion IMP3 may be an effective and useful mo- lecular maker for diagnosis of benign and malignant thyroid neoplasms, as well as the differential diagnosis between benign and malignant thyroid tollicular lesions.