摘要
为了研究厚朴总酚对乳房炎致病菌(主要为金黄色葡萄球菌)的抗菌活性和抗感染机制,首先通过肉汤稀释法检测厚朴总酚对金黄色葡萄球菌的最小抑菌浓度,通过溶血试验、蛋白质免疫印迹试验、荧光定量PCR试验和LDH释放试验从不同层次和角度验证了厚朴总酚对金黄色葡萄球菌致病力的影响。结果显示,厚朴总酚对金黄色葡萄球菌具有一定的抗菌活性,其对金黄色葡萄球菌最小抑菌浓度范围为8~16μg·mL-1。另外,本研究结果表明亚抑菌浓度的厚朴总酚可抑制α-溶血素的表达从而降低金黄色葡萄球菌的致病力,并可明显缓解金黄色葡萄球菌α-溶血素介导的细胞的损伤作用。
In order to investigate the antimicrobial activity and anti-infective activity of Total Magnolol on mastitis-related pathogens（mainly Staphylococcus aureus）,a broth microdilution method was employed to determine the minimal inhibitory concentrations（MIC）of the Total Magnolol against S.aureus strains.Hemolysis assay,Western blot analysis,real-time RT-PCR assay and cytotoxic assay were further performed to evaluate the influence of this compound on S.aureus virulence.The results showed that the minimal inhibitory concentration of Total Magnolol against S.aureus were 8-16μg·mL^-1.Furthermore,treatment with Subinhibitory concentration of Total Magnolol could inhibit the expression ofα-hemolysin,reduce the pathogenicity of Staphylococcus aureus,and significantly alleviate the injury induced byα-hemolysin of Staphylococcus aureus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第2期432-438,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31130053)
天津市现代农业产业技术体系创新团队建设专项(ITTCRS2017008)
