HPLC法同时测定外搽白灵酊中7种成分

Simultaneous determination of seven constituents in Waicha Bailing Tincture by HPLC

目的建立HPLC法同时测定外搽白灵酊(白芷、苏木、红花等)中巴西苏木素、(±)原苏木素B、羟基红花黄色素A、红花黄色素A、氧化前胡素、欧前胡素、异欧前胡素的含有量。方法该药物甲醇提取液的分析采用Agilent TC-C18色谱柱(200 mm×4.6 mm,5μm);以甲醇-0.1%冰醋酸为流动相,梯度洗脱;检测波长285、403、310 nm;体积流量0.9 m L/min;柱温25℃。结果 7种成分在各自范围内线性关系良好(r≥0.999 5),平均加样回收率96.91%~99.59%,RSD 0.81%~1.19%。结论该方法稳定可靠,可用于外搽白灵酊的质量控制。

AIM To eslablish an HPLC method for the simultaneous content determination of brazilin,（ ±）protosappanin B,hydroxysafflor yellow A,safflor yellow A,oxypeucedanin,imperatorin and isoimperatorin in Waicha Bailing Tincture（ Angelicae dahuricae Radix,Sappan Lignum,Carthami Flos,etc.）. METHODS The analysis of methanol extract of this drug was performed on a Agilent TC-C18 column（ 200 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of methanol-0. 1% glacial acetic acid flowing at 0. 9 m L/min in a gradient elution manner,and the detection wavelengths were set at 285,403,310 nm. RESULTS Seven constituents showed good linear relationships within their own ranges（ r≥0. 999 5）,whose average recoveries were 96. 91%-99. 59%with the RSDs of 0. 81%-1. 19%. CONCLUSION This stable and reliable method can be used for the quality control of Waicha Bailing Tincture.