紫地榆HPLC指纹图谱建立及模式识别

Establishment of HPLC fingerprints of Geranium strictipes and pattern recognition

目的建立紫地榆Geranium strictipes R.Kunth HPLC指纹图谱,并进行模式识别。方法紫地榆70%甲醇提取液的分析采用Aglient ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm);乙腈-水为流动相,梯度洗脱;柱温25℃;检测波长254 nm。对结果进行聚类分析、主成分分析。结果 21批样品HPLC指纹图谱中有13个共有峰,相似度在0.894以上。21批样品分为2类,第一主成分的贡献率31.067%。结论该方法稳定、可靠、重复性好,可用于紫地榆的质量控制。

AIM To establish HPLC fingerprints of Geranium strictipes R. Kunthand to perform pattern recognition. METHODS The analysis of 70% methanol extract of G. strictipes was performed on a 25℃ thermostatic ZORBAX SB-C18 column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile-water in a gradient elution manner,and the detection wavelength was set at 254 nm. The results were analyzed by cluster analysis and principal component analysis. RESULTS There were thirteen common peaks in the HPLC fingerprints of twenty-one batches of samples with the similarities of more than 0. 894. Twenty-one batches of samples were divided into two categories. The cumulative contribution rate of the first principal component was 31. 067%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G. strictipes.