UPLC法测定逐瘀通窍口服液中10种有效成分的含量

Simultaneous determination of 10 active compounds in Zhuyutongqiao oral liquid by UPLC method

目的建立超高效液相色谱法同时检测逐瘀通窍口服液中原儿茶醛、苦杏仁苷、芍药苷、阿魏酸、甘草苷、柚皮苷、橙皮苷、新橙皮苷、甘草酸铵和α-细辛醚10种成分的含量。方法采用HSS T3色谱柱(ODS,C_(18),100 mm×2.1 mm,1.8μm),以乙腈-体积分数0.4%磷酸水溶液为流动相,梯度洗脱,流速为0.3 m L·min^(-1),柱温为30℃,检测波长为210 nm,进样量为2μL。结果 10种有效成分均呈良好线性关系,r均大于0.999,方法精密度、重复性RSD均小于2.0%,24 h内稳定性良好,平均加样回收率为97.63%~104.09%,RSD均小于2.0%。结论所建立的UPLC方法可用于逐瘀通窍口服液的质量控制。

Objective To establish a UPLC method for determination of ten active compounds in Zhuyutongqiao oral liquid,including protocatechuic aidehyde, amygdalin, peoniflorin, femlic acid, liquiritin, naringin, hesperidin, neohesperidin, ammonium glycyrrhetate and ot-asaron. Methods The chromatographic fingerprint was obtained gradient eluted with acetonitrile-0.4% phosphate acid solution at the flow rate of 0.3 mL·min^-1 and on an acquity UPLC HSS T3 column（ODS C18,100 mm×2.1 mm,1.8 μm）. The detection wavelength was set at 210 nm. The column temperature was maintained at 30^（2. Results The concentrations had a linear relationship with the peak areas of the 10 compounds was （ r 〉 0. 999 ）. The precision and reproducibility were good（ RSD less than 2.0% ,respectively）. The sample solution was stable in 24 h （ RSD 〈 2.0% ）. The average recoveries were all within 97.63% -104.09% （ RSD 〈 2.0% ）. Conclusions The determination is convenient, fast and accurate, and it could be used for the quality control of Zhuyutongqiao oral liquid.