参知健脑胶囊对脑缺血大鼠局部脑血流量的影响

Shenzhi Jiannao Capsules on local cerebral blood flow in rats with cerebral ischemia

目的观察参知健脑胶囊对大鼠局部脑血流量的影响。方法将60只SD大鼠随机分为假手术组,模型组,金纳多组,参知健脑胶囊低、中、高组,每组10只。将大鼠囟门处颅骨磨薄,然后采用结扎双侧颈总动脉建立低灌注模型,术后10 min,除假手术组和模型组给予等体积生理盐水外,其余各组直肠注射给药,分别给予金纳多20 mg/kg以及参知健脑胶囊40、120、360 mg/kg。应用激光多普勒血流仪检测各组给药后30、60、90、120 min时点动物rCBF变化。结果模型组术后rCBF下降明显,与假手术组相比有显著统计学差异(P<0.01);参知健脑胶囊在给药后30 min^120 min可明显抑制脑缺血大鼠rCBF的下降,与模型组比较差异显著(P<0.05,P<0.01),与金纳多组相比无显著差异(P>0.05)。结论参知健脑胶囊抑制脑缺血大鼠rCBF的疗效与金纳多相似,其治疗血管性痴呆的作用可能与其改善脑部缺血状态有关。

Objective To observe the effect of Shenzhi Jiannao Capsules on local cerebral blood flow in rats.Methods 60 SD rats were randomly divided into sham-operated group,model group,Ginaton and Shenzhi Jiannao Capsules low,middle,high dosage groups,10 rats each group.First,grinding the skull on fontanelle to make it thinner,then using bilateral comman carotid artery occlusion to establish hypoperfusion model.10 minutes after the operation,except sham-operated group and model group were given equal volume of normal saline,the other groups were given Ginaton 20 mgkg and Shenzhi Jiannao Capsule 40 mg/kg、120 mg/kg、360 mg/kg by intrarectal administration.Laser Doppler Flowmetry was used to detect rCBF of each group.Results After operation,rCBF of model group was dramaticly decreased compared to sham-operation group and had significant statistical difference（P 〉0.01）; 30 minutes to 120 minutes after administration,Shenzhi Jiannao Capsules could inhibit the decrease of rCBF and had statistical difference compared to model group（P〈0.05-0.01）,while there was no statistical difference compared to Ginaton group（P〉0.05）.Conclusion Shenzhi Jiannao Capsules had similar effect as Ginaton in inhibiting the decrease of rCBF of ischemic rats,and its efficacy on vascular dementia may be related to the improvement of brain ischemia state.