基于自然加倍为主体的DH双轮回选择玉米育种技术体系的构思

Conception of New Maize Breeding System Based on Spontaneous Doubling Capabilities Integrated into Recurrent Selection Scheme

依据相关理论和育种实践提出,在单倍体规模化育种技术基础上,应用单倍体轮选遗传修复原理,纳入主体杂优模式双向轮回选择育种理念,融入分子辅助设计、数据采集与分析处理等现代技术,构建＂基于自然加倍为主体的单倍体双轮回育种集成技术体系＂。通过对主体杂优模式的双向DH轮回循环选择改良,逐步累加农艺性状相关有益基因频率,快速聚合雄穗自然加倍基因,随育种进程逐步深入,形成两个相互对应的单倍体高自然加倍商业化核心种质群,逐步摆脱复杂苛刻的实验室或工厂化加倍技术环节,步入简捷高效的单倍体田间自然加倍技术体系。该体系能够实现育种技术全新跨越,大幅度提高育种效率,缩短育种年限,简化操作流程。

Based on related theory and breeding practice, at the foundation of scaled haploid breeding tech-nique, the genetic repairing mechanism of the haploids was applied to recover its doubling capabilities in recurrentselection scheme in this article. We are integrated this recovering doubling capabilities into the main frame of the bi-directional heterotic group breeding practice using recurrent breeding selection concept, and also incorporated intomodern technologies of molecular assisted design, and data collection and analysis, to construct a＂double recurrentbreeding integrated technology system of the haploids, mainly to enhance the spontaneous doubling rate.＂Throughthe improvement of recovering haploids doubling rate into the main frame of bidirectional heterotic group recurrentbreeding scheme, we are able to rapid accumulate spontaneous doubling genes of the male tassel, and also cumula-tively to combine useful genes together for useful agronomic traits improvement. Follow the progression of breeding,we are able to generate two high haploid doubling commercial core germplasm heterotic groups of male and female.These two source materials help to give high haploid doubling rate, to help to bypass complicate and toxic laboratoryor industrialized chromosome doubling procedures, and to help to establish a very simplified and high throughput＂field haploid spontaneous doubling technology system＂. This improved system can accomplish new crossing ofbreeding technology, greatly improve breeding efficiency, reduce breeding time, and simplify breeding procedures.