牛磺酸对锰致大鼠空间记忆能力衰退的影响

The Effect of Taurine in Improving Spatial Memory Ability of Rats Affected by Manganese

目的:探讨牛磺酸对锰致大鼠空间记忆能力衰退的改善作用。方法:选取健康SD雄性大鼠42只,随机均分为对照组、锰组、牛磺酸组;锰组和牛磺酸组腹腔注射氯化锰溶液15 mg/(kg·d),每周5 d,共3周,牛磺酸组同期皮下注射牛磺酸溶液200 mg/(kg·d)每周5 d,共3周,对照组同期腹腔注射0.9%Na Cl溶液30 mg/(kg·d);Morris水迷宫记录各组大鼠的空间探索时间及搜索次数,以评价大鼠的空间记忆能力;行为学实验后取各组大鼠海马,检测谷氨酸相关酶、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及超氧化物歧化酶(SOD)水平。结果:与对照组相比,锰组逃避潜伏期、游泳总路程均明显延长(P<0.05),谷氨酰胺合成酶、谷氨酰胺酶、谷氨酸脱羧酶明显减少(P<0.05),MDA含量、GSH-Px活性明显增加,SOD含量明显减少(P<0.05);与锰组相比,牛磺酸组逃避潜伏期、游泳总路程均明显缩短(P<0.05),谷氨酰胺合成酶、谷氨酰胺酶、谷氨酸脱羧酶明显增多(P<0.05),MDA含量、GSH-Px活性明显减少,SOD含量明显增加(P<0.05)。结论:牛磺酸减少锰致大鼠的神经细胞损害,改善了空间记忆能力,减少了氧化损伤。

Objective： To investigate the effect of taurine in improving the spatial memory ability of rats that affected by manganese. Methods： Forty-two healthy SD male rats were chosen and randomly divided into control group （ group C ）, manganese group （ group M ）, and taurine group （ group T ） with 14 in each group. Rats in groups M and T were injected intra-peritoneally with manganese chlo- ride solution 15 mg/（kg · d） or taurine 200 mg/（kg·d） respectively, five days a week fi）r 3 weeks, while rats in group C were injected with 0.9 NaCl solution 30rag/（ kg· d） instead tot the same course. Morris water maze test and diving tower test were performed to evaluate special memory ability. Finally, hippocampus of rats were taken out and glutamine-related enzymes and MDA, gsh-px and SOD levels were detected. Results： Compared with those in group C, the escape latency and swim- ming distance in group M were significantly longer （P 〈 0.05） , and the glutamine synthetase, glutam- inase, glutamic acid decarboxylase in group M decreased significantly （P 〈 0.05）. In group M, MDA contents and GSH-Px activity were higher and SOD content was lower than those in group C significant- ly （P 〈 0.05）. Compared with those in group M, escape latency and swimming total distance in group T were obviously shorter （ P 〈 0.05 ）. The glutamine synthetase, glutaminase, glutamic acid decarbox- ylase and SOD content in group T were significantly higher than those in group M （P 〈 0.05 ）, while the MDA content and GSH-Px activity of group T was obviously lower than those in group M. Conclu- sion： Taurine could reduce damage in nerve cells of rats, improve spatial memory and reduce oxidative damage.