正交设计优化黄芪多糖提取工艺

Optimization of Extraction Technology of Astragalus Polysaccharide by Orthogonal Design

[目的]优化黄芪多糖的提取工艺。[方法]以料液比、提取时间、提取次数、浸泡时间为试验因素,各因素分别设计3个水平,采用L_9(3~4)正交设计,以黄芪多糖含量为考察指标,优选黄芪多糖的水回流提取工艺。[结果]最佳提取工艺条件为:料液比1∶12,提取时间1.0 h,浸泡时间1.0 h,提取次数3次。在最佳提取工艺条件下,黄芪多糖含量占原药材的6.4%,多糖含量占粗多糖的58.23%。[结论]优化后的提取工艺操作简便、快捷,重复性、稳定性良好。

[objective]To optimize the extraction technology of Astragalus polysaccharide. [Methods]The solid to liquid ratio,extraction duration, extraction times, and immersion duration were chosen as the experimental factors in Astragalus polysaccharide extraction, and there were three levels for each of the factor. The water reflux extraction technology of Astragalus polysaccharide was optimized by L_9（3~4） orthogonal test using Astragalus polysaccharide content as assessing index. [Results]The optimum extraction conditions were as followed： solid to liquid ratio was 1∶12, extraction duration was 1.0 h, extraction repeated 3 times, and immersion duration was 1.0 h. Under these conditions, the obtained Astragalus polysaccharide content accounted for 6.4% and 58.23% of the raw medicinal materials and crude polysaccharide, respectively. [Conclusion]The optimized extraction technology of Astragalus polysaccharide is simple and quick to operate and has good repeatability and stabilization.