不同微生物处理玉米胚芽粕对瘤胃体外发酵参数的影响

Effects of Treatment of Corn Germ Meal by Different Microbes on the Rumen Fermentation Parameters in vitro

为探究不同微生物处理玉米胚芽粕对瘤胃体外发酵参数的影响,试验选用高产细菌素乳酸菌、酿酒酵母菌、枯草芽胞杆菌3种微生物及其组合对玉米胚芽粕进行发酵处理,每个处理5个重复;选出发酵效果较佳的混菌组与原料采用体外发酵法测定其产气量(GP)、p H值、干物质降解率(DMD)、氨态氮(NH_3-N)。结果表明:(1)与对照组相比,各处理组粗蛋白含量显著提高(P<0.05),混菌组真蛋白含量显著提高(P<0.05),各处理组中性洗涤纤维显著下降(P<0.05)。(2)混菌组2~48 h的累积产气量均显著高于对照组(P<0.05),混菌组产气曲线的平滑度B值、达到理论产气量最大值一半所需时间C值显著高于对照组(P<0.05)。(3)混菌组在48 h的p H值显著低于对照组(P<0.05);混菌组干物质降解率、氨态氮浓度显著高于对照组(P<0.05)。说明微生物处理玉米胚芽粕能提高该原料的营养价值,而混菌处理效果更佳,混菌发酵处理可提高反刍动物对其利用价值。

To explore the effects of treatment of corn germ meal by different microbes on the fermentatinn parameters of rumen invitro,high bacteriocin producing lactic acid bacteria,yeast,bacillus subtilis and its combination were processed on corn germmeal, each with 5 replicates;then GP, pH, DMD, NH3-N of the mixed bacteria group with better effect and the raw materials weredetermined. The results showed that ： compared with the control group,CP of each treatment group increased significantly （P〈0.05） ,TP of the mixed bacteria group was significantly increased （P〈0.05）,and NDF of＂ each treatment group decreasedsignificantly （P〈0.05）.GP of 2-48 h in the mixed bacteria group was significantly higher than control group （P〈0.05）.B value（tile smoothness of the gas production cnrve）aud C value （time of the maximum value of theoretical gas production）weresignificantly higher than control group （P〈0.05）. pH of the mixed bacteria group in 48 h was significantly lower than controlgroup （P〈0.05）,DMD and NH3-N in the mixed bacteria group were significantly higher than control group （P〈0.05）. Inconclusion,microbial treatment of corn germ meal can irnprove the nutritional value of the raw material,while effect of mixedbacteria treatment is better. Mixed bacteria treatment can improve the utilization value of corn germ meal by ruminants.