脂肪因子Chemerin促进RF/6A细胞增殖、迁移和管腔形成

Research on the Effect of Chemerin on RF/6A Cell Proliferation, Migration and Tube Formation

目的：研究Chemerin对体外培养的恒河猴脉络膜视网膜血管内皮细胞（RF/6A细胞）增殖、迁移和管腔形成的影响。方法：实验研究。取体外培养的生长良好的RF/6A细胞用于实验，将细胞随机分为4组，分别是对照组、5 ng/ml浓度Chemerin组、10 ng/ml浓度Chemerin组、20 ng/ml浓度Chemerin组。在RPMI-1640 培养液中进行培养24、48 h后，采用MTT法检测细胞增殖，划痕法检测细胞迁移，基质胶法检测细胞管腔形成。多组间比较采用单因素方差分析。结果：MTT检测结果提示，同对照组比较，10、20 ng/ml浓度Chemerin能够显著促进细胞增殖，组间差异均有统计学意义（P ＜0.05），5 ng/ml浓度组与对照组差异无统计学意义；划痕法检测细胞迁移结果提示，同对照组比较，3 种Chemerin浓度均能够显著促进细胞迁移，呈剂量依赖性，组间差异均有统计学意义（P ＜0.05）；基质胶法检测细胞管腔形成结果提示，同对照组比较，3 种Chemerin浓度均能够显著促进细胞管腔形成，呈剂量依赖性，组间差异均有统计学意义（P ＜0.05）。结论：脂肪因子Chemerin能够促进RF/6A细胞增殖、迁移和管腔形成，提示Chemerin在视网膜新生血管形成中起重要作用。

Objective： To investigate the effects of chemerin on the proliferation, migration and tube formation of choroid retinal vascular endothelial cells （RF/6A） in the rhesus monkey in vitro. Methods： In this experimental study, RF/6A cells were randomly divided into 4 groups： a control group, and 3 chemerin groups treated with concentrations of 5, 10 or 20 ng/ml. The cells were cultured in a RPMI-1640 medium.After 24 h and 48 h in the culture, the MTT method was used to detect RF/6A cell proliferation. Cellmigration was detected by scratch test, and cell tube formation was detected by matrigel. A one-way analysis of variance was used to compare the data of the multiple groups. Results： MTT results showed that 10 ng/ml and 20 ng/ml concentrations of chemerin significantly promoted cell proliferation compared to the control group （P 〈 0.05）. There was no significant difference between the 5 ng/ml concentration group and the control group. Cell migration results showed that the three different concentrations of chemerin significantly promoted cell migration in a dose-dependent manner. The difference between groups was statistically significant （P 〈 0.05）. The three different concentrations of chemerin significantly promoted cell tube formation in a dose-dependent manner （P 〈 0.05） in the treated groups compared to the control group. Conclusions： Chemerin can promote the proliferation, migration and tube formation of RF/6A cells, suggesting that chemerin may play an important role in retinal neovascularization.