应用竞争力指数分析Ⅲ型效应子SKWP对青枯菌在寄主植物体内增殖能力的影响（英文）

Analysis of the SKWP effectors to bacterial fitness in host plant by a novel competition assay

【目的】研究Ⅲ型效应子SKWP对青枯菌OE1-1在寄主植物体内增殖能力的影响。【方法】构建青枯菌RK7197(野生型突变体,带Gm抗性)和SKWP单基因缺失突变体(带PB抗性),通过竞争力指数分析SKWP各效应子对青枯菌OE1-1在叶片组织内增殖能力的影响。【结果】竞争力指数适合在寄主植物茄子上分析各效应子功能,6个SKWP效应子对OE1-1细菌增殖能力影响不同,SKWP4影响最明显。【结论】竞争力指数可提供一个新视野来分析SKWP各效应子对青枯菌OE1-1在寄主茄子上增殖能力的影响。

[Objective] By testing the internal bacterial growth of SKWP mutants generated in Ralstonia solanacearum strain OEI-1, we evaluated the contribution of these SKWP effectors to bacterial fitness towards host plants. [Methods] The deletion mutant and complementation mutant of R. solanacearum OE 1-1 were constructed. A competition assay in mixed infection was adopted to evaluate the contribution of the SKWP to bacterial fitness in tobacco （Nicotiana tabacum cv. Bright Yellow） and eggplant （Solarium melongena cv. Senryo-nigou）. [Results] Eggplant was the appropriate host for competitive index （CI） assay of R. solanacearum strain OEI-1. All 6 SKWP mutants affected bacterial fitness in eggplant to some extent while SKWP4 appeared to be most important. [Conclusion] The SKWP effectors were important for bacterial proliferation in eggplant tissues according to the CI analysis, which paving a new way for further identification of the function on whole SKWP family.