灵芝三萜液态深层发酵的研究进展

Research Progress on Ganoderma triterpenes Obtained by Liquid Submerged Fermentation

三萜类化合物是灵芝中主要的活性化学成分之一,由于其具有多种生理活性,现已成为灵芝研究开发的热点。为了给灵芝三萜液态深层发酵的调控及未来的规模化生产提供参考和借鉴,本研究聚焦通过发酵工程手段获得灵芝三萜的最新研究工作,综述了灵芝发酵方法中三萜的检测方法、发酵技术、液态深层发酵动力学及与灵芝三萜生物合成相关途径等方面的研究进展。在此基础上指出了研究中存在的问题:没有系统性地筛选适合发酵工程生产的灵芝三萜菌株,缺乏对菌丝体中三萜的化学结构和药理活性方面的研究,目前只有较少规模化的利用发酵法生产灵芝三萜的研究,缺少直接利用活性追踪的方法优化灵芝三萜生产工艺等,最后进行了展望。

Triterpenes are one of the main active chemical components in Ganoderma, and it has become thehotspot in current research due to multiple physiological activities. In order to provide a reference for the liquidsubmerged fermentation regulation of Ganoderma triterpenes and large-scale production, we focus on therecent latest research of Ganoderma triterpenes produced by fermentation engineering. In addition, the progressof the detection method of triterpenes, fermentation technology, fermentation kinetics, and the triterpenesbiosynthesis pathway was reviewed. On this basis, the problems in research were as follows： we had notscreened Ganoderma triterpenes strains systematically suitable for the fermentation production, we lacked thestudy of triterpenes chemical constitution and pharmacological activity, there was only less study on the Ganoderma triterpenes large-scale production by fermentation, and we lacked the production engineering of Ganoderma triterpenes optimized by activity tracking. At last, the progress was forecasted.