不同品系橡胶树花药愈伤诱导和体胚发生研究

Induction of Anther Callus and Somatic Embryogenesis of Different Rubber Tree(Hevea brasiliensis Muell.Arg.) Clones

为建立不同品系橡胶树的花药愈伤诱导和体胚发生体系,以橡胶树优良品系‘热研106’、‘热试59’、‘热试62’的花药为材料,采用正交设计,对不同品系、不同植物生长调节剂配比培养基的花药愈伤和体胚诱导效果进行了研究。结果表明:3个品系的愈伤诱导率和体胚诱导效率差异明显,同一品系不同培养基的愈伤诱导率和体胚诱导效率差异明显;愈伤诱导培养基中的植物生长调节剂对下一阶段的体胚诱导有明显影响。‘热研106’的花药愈伤诱导最佳浓度组合为2,4-D 1 mg/L+NAA 2 mg/L+KT 1 mg/L,‘热试59’的花药愈伤组织诱导最佳浓度组合为2,4-D 0.5mg/L+NAA 1 mg/L+KT 1 mg/L,‘热试62’的花药愈伤组织诱导最佳浓度组合为2,4-D 1 mg/L+NAA 1 mg/L+KT 1 mg/L。‘热试62’的体胚发生最佳浓度组合为KT 3 mg/L+NAA 0.05 mg/L+GA30.05 mg/L+6-BA 0.5 mg/L。本研究获得了不同品系的愈伤诱导和体胚发生最佳培养基组合。

In order to establish the system of anther callus induction and embryogenesis of different rubber tree strains,we take the anther of rubber tree ＇Reyan106＇,＇Reshi59＇,＇Reshi62＇ as the research material,using orthogonal design,and studied the effects of different clones and the different plant growth regulator ratio mediums on callus and somatic embryo induction.The results showed that the callus induction rates（CIRs）and embryogenesis induction efficiency（EIE） of three strains were very different,and the CIRs and EIE of one strain under different mediums were also very different.The plant growth regulators in the callus induction medium had an obvious effect on the subsequent somatic embryo induction.For callus induction,2,4-D 1 mg/L＋NAA 2 mg/L＋KT 1 mg/L was the best for‘Reyan 106＇,and 2,4-D 0.5 mg/L＋NAA 1 mg/L＋KT 1 mg/L was the optimum for‘Reshi 59＇,and 2,4-D 1 mg/L＋NAA 1 mg/L＋KT 1 mg/L was most suitable for‘Reshi 62＇.For somatic embryo induction,KT 3 mg/L ＋ NAA 0.05 mg/L ＋ GA3 0.05 mg/L ＋ 6-BA 0.5 mg/L was optimal for‘Reshi 62＇.In the present study,the best medium of callus induction and somatic embryogenesis for different strains was obtained.