密蒙花中黄酮类成分的分离与鉴定

Isolation and identification of flavonoids from Buddleja officinalis flos

目的对密蒙花(Buddleja officinalis Maxim.)中的黄酮类成分进行研究,为其资源开发与利用提供依据。方法综合应用大孔吸附树脂、正相硅胶、反相ODS和Sephadex LH-20等柱色谱以及高效液相色谱法对密蒙花中黄酮类成分进行分离纯化,并结合单体成分的理化性质与核磁共振波谱数据鉴定其结构。结果与结论从密蒙花体积分数70%乙醇溶液的提取物中分离鉴定了11个黄酮类化合物,分别为木樨草素(1)、木樨草素-7-O-葡萄糖苷(2)、木樨草素-7-O-芸香糖苷(3)、芹菜素-7-O-β-D-葡萄糖苷(4)、芹菜素-7-O-β-D-芸香糖苷(5)、蒙花苷(6)、芹菜素-7-O-β-D-葡萄糖醛酸苷(7)、芹菜素-4'-O-β-D-葡萄糖醛酸苷(8)、芹菜素-7-(2'-α-L-鼠李糖基)芸香糖苷(9)、密蒙花新苷(10)、山柰酚-3-O-芸香糖苷(11),其中,化合物7~9、11均为首次从醉鱼草属植物中分离得到。

To promote the development of Buddleja officinalis Maxim. , the flavonoid glycosides from it had been studied. Through the usage of multiple chromatographic methods such as D101 resin, silica gel, Sephadex LH-20, ODS and HPLC, eleven flavonoid glycosides had been isolated. Combining the physicochemical properties with spectral data, their structures were elucidated as luteolin（ 1 ）, luteolin 7-0-β- D-glucopyranoside （ 2 ）, luteolin-7-O-β-D-rutinoside （ 3 ）, apigenin-7-O-β-D-glucopyranoside （ 4 ）, apigenin-7- O-rutinoside （5）, linarin （ 6 ）, apigenin-7-O-β-D-glucuronide （ 7 ）, apigenin-4＇-O-β-D-glucuronide （ 8 ）, apige- nin-7-（2＇-rhamnosyl） rutinoside （ 9 ）, neobudofficide （ 10 ）, and kaempferol-3-O-β-D-rutinoside （ 11 ）, respectively. Among them, compounds 7 -9,11 were obtained from Buddleja genus for the first time.