落叶松热解油防霉抑菌特性研究

Antimidew and Antibacterial Characteristics of Larix Bio-oil

为了评价热解油的防霉抑菌性能,在利用GC-MS分析落叶松热解油主要挥发性化学成分的基础上,采用滤纸片法和琼脂稀释法测试落叶松热解油对3种木材常见霉菌(黑曲霉菌、桔青霉菌和绿色木霉菌)的防霉抑菌性能。结果表明:落叶松热解油中含有酚类(34.59%)、酮类(17.53%)、醛类(11.31%)、酯类(6.66%)、醇类(6.55%)和有机酸类(6.04%)等6大类物质。落叶松热解油对黑曲霉菌、桔青霉菌和绿色木霉菌的抑菌环直径平均值分别达到15.67、19.67和14.28 mm,且3种霉菌的阴性对照样片均无抑菌环产生,表明落叶松热解油具有一定的防霉抑菌效果;随着热解油体积分数的增加,对3种霉菌的抑菌率均增大,对3种霉菌的最低抑菌质量分数均为25%。

To evaluate the antimidew and antibacterial characteristics of bio-oil, the main volatile chemical components of Lar/x bio-oil were analyzed by GC-MS, and the filter paper and agar dilution methods were used to evaluate their antimidew and antibacterial effects on three common wood molds （Aspergillus niger, Penicillium citrinum and Trichoderma viride）. The results showed that the Lar/x bio-oil contained a variety of antimicrobial components, such as phenols（ 34. 59 % ）, ketones（ 17. 53 % ）, aldehydes（ 11.31%）, esters（6.66 %）, alcohols（6.55 %）, organic acids（6. 04 %）, and etc. The maximum diameters of the bacteriostatic circles of Larix bio-oil for A. niger, P. citrinum and T. viride were 15.67, 19. 67 and 14. 28 mm, respectively and the bacteriostatic circles were not found in the contrast samples indicated that the Larix bio-oil has good bacteriostatic effect. With the increase of bio-oil volume fraction, the inhibitory rates of 3 kinds of fungi increased, the minimum inhibitory concentration of three kinds of mould was 25% by agar dilution method.