条斑紫菜杂交重组品系(A-18)的筛选与特性分析

Selection and characterization of an improved strain(A-18)by hybridization recombinant in Pyropia yezoensis(Bangiales,Rhodophyta)

为筛选出藻体生长快且颜色与野生型色泽相近的条斑紫菜新品系,本研究从条斑紫菜绿色突变体和红色突变体种内杂交产生的后代中,分离出了优良品系A-18。日龄60d时的叶状体平均长度、长宽比和单株湿质量,A-18品系分别为84.95cm、49.46和0.52g,分别是条斑紫菜野生型品系(WT)的3.12、7.01和1.36倍。日龄60d时叶状体的叶绿素a和总藻胆蛋白含量,A-18品系分别为8.37和53.81mg/g,均与WT品系较接近。日龄60d时的叶状体平均厚度,A-18品系仅为20.22μm,比WT品系降低了29%。另外,A-18品系的壳孢子放散总量为916.01万个/贝壳,是WT品系的1.55倍。综上所述,A-18品系具有生长快、长宽比值大、藻体薄、壳孢子放散量大等优良特性,且藻体颜色与野生型色泽相近,有望在生产中运用。

In order to select a new variety of Pyropia yezoensis with fast growing and wild-type color,following intraspecific hybridization of the green-type pigmentation mutant and red-type pigmentation in P.yezoensis was carried out,and then an improved strain（A-18）was isolated from the recombinants.The mean length,mean lengthwidth ratio and wet weight of 60-day-old blades in A-18 strain were 84.95 cm,49.46 and 0.52 g,which were 3.12,7.01 and 1.36 times those of WTstrain.After being cultured for 60 days,the contents of chlorophyll aand phycobiliprotein in A-18 strain were 8.37 and 53.81 mg/g,respectively,both of them were close to the WTstrain.The thickness of 60-day-old blades of A-18 strain was 20.22μm,which was 29%thinner than that of WTstrain.In addition,the total number of conchospores released fromA-18 strain was 916.01×104 per shell,which was 1.55 times that of WTstrain.The above results showed that A-18 strain was characterized by fast growth,large lengthwidth raito,large amount of conchospores,and its color is close to the wild-type,therefore,A-18 strain is expected to be applied in the production.