桂皮总黄酮的纯化工艺及其抗氧化性研究

Purification technology of total flavonoids in cinnamon with macroporous resin and its antioxidant ability

探索大孔吸附树脂纯化桂皮总黄酮的工艺条件及其体外抗氧化活性。以总黄酮的吸附量、吸附率及解吸量、解吸率为考察指标,采用单因素实验对桂皮总黄酮的纯化工艺进行考察;采用分光光度法探索了桂皮总黄酮对DPPH·的清除活性。研究结果表明,LSA-21型大孔吸附树脂对桂皮总黄酮纯化的较佳工艺为:提取液总黄酮质量浓度为0.7 mg/mL,桂皮提取液的上样体积与树脂质量之比(mL:mg)为12:1,吸附流速控制为1.0 mL/min,解吸液为50%乙醇水溶液,解吸流速控制为1.5 mL/min,解吸液体积与树脂质量之比(mL:mg)为8∶1。在上述纯化工艺条件下,桂皮提取液经LSA-21型大孔吸附树脂纯化后,干浸膏中总黄酮含量由18.74%提高到43.77%,桂皮采用石油醚脱脂后,干浸膏中总黄酮含量可以提高到48.97%。体外抗氧化活性的实验结果表明,桂皮总黄酮对DPPH·具有清除活性,而且随着质量浓度的增加,清除活性明显增强。研究结果为桂皮的进一步开发利用提供了依据。

The purification condition of total flavonoids from cinnamon by macroporous resin and its antioxidant activity in vitro was studied. The purification process of TFs was optimized by single factor test and absorbance,adsorption ratio and adsorption quantity,desorption ratio were the indexes. The scavenging activity of total flavonoids from cinnamon on DPPH · was studied by spectrophotometry. The results showed that the optimum purification conditions of TFs in cinnamon were as follows ：The extraction of total flavonoids concentration 0.7 mg/mL,the ratio of sample volume of the extraction with resin mass（mL ： mg）12 ∶ 1,the adsorption velocity 1.0 mL/min;the eluate ethanol solution 50%（volume fraction）,the desorption velocity 1.5 mL/min,the ratio of desorption volume with resin mass（mL ： mg）was 8 ：1. Under the conditions of purification,the purity of TFs in cinnamon extracts could be changed from 18.74% to 43.77%. After using petroleum ether degreasing,the purity of TFs in cinnamon extracts improved to 48.97%. The activity test showed that total flavonoids from cinnamic had strong scavenging activity to DPPH ·,and the scavenging activity might gradually enhanced with the increase of mass concentration of total flavonoids in extraction solution. The results provide basis for further development and utilization of cinnamon.