补骨脂异黄酮对β-淀粉样蛋白损伤PC12细胞的影响

Effects of psoralen isoflavones on the treatment of PC12 cells injured by Aβ

目的 研究补骨脂异黄酮对β-淀粉样蛋白（β-amyloid protein, Ab）损伤PC12细胞相关蛋白表达的影响，探讨其作用机制。方法 将PC12细胞按随机数字表法分为空白组、模型组、E2组、补骨脂异黄酮组，除空白组其余各组培养液中均加入20 μmol/L的Ab25-35进行造模，E2组加入10-3μmol/L的雌激素及补骨脂异黄酮组加入10～10^-1 μmol/L的补骨脂异黄酮干预。采用MTT法检测PC12细胞增殖率，并利用Western Blot法检测b淀粉样前体蛋白（β-amyloid precursor protein, APP）、b位淀粉样裂解酶-1 （β-site amyloid cleavage enzyme 1, BACE1）、雌激素受体b（estrogen receptor b, ERb）、磷酸化细胞外信号调节蛋白激酶（phosphorylated extracelluar signal-regulated kinase, p-ERK）及Ab的表达量。结果 10-1 mmol/L的补骨脂异黄酮对PC12细胞增殖率（101%比52%）水平升高（P＜0.01）；p-ERK[（0.751±0.066）比（0.364±0.015）]、ERb[（0.756±0.105）比（0.337±0.045）]表达量均明显升高（P＜0.01）；APP[（0.382±0.039）比（0.479±0.015）]、BACE1[（0.517±0.024）比（0.622±0.029）]、Ab[（0.430±0.032）比（0.581±0.030）]表达量均明显降低（P＜0.05）。结论 补骨脂异黄酮对Ab损伤PC12细胞有一定的保护作用。

Objective To study the effect of psoralen isoflavone on the treatment of PC12 cells injured by Ab and the mechanism on the effect of the psoralen isoflavones on the expression of related proteins. Methods The PC12 cells were divided into blank group, model group, E2 group, and psoralen isoflavones group by random number table method, In addition to the blank group the rest of each group culture medium were added 20μmol/L of Aβ25-35 modeling, The E2 group was added to the 10-3μmol/L oestrogen and psoralen isoflavones group for the intervention of 102-10-6 μmol/L.The proliferation rate of PC12 cells was detected by MTT assay, and the expression of APP, BACE1, ERβ, p-ERK and Aβ protein was detected by Western Blot. Results Compared with the model group, the proliferation of PC12 cells induced by 10-1μmol/L of psoralen isoflavone increased （101% vs. 52%, P〈0.01）; The expression of p-ERK （0.751± 0.066 vs. 0.364 ± 0.015）, ERβ （0.756 ± 0.105 vs. 0.337 ± 0.045） increased significantly （P〈0.01）; APP （0.382 ± 0.039 vs. 0.479 ± 0.015）, BACE1 （0.517 ± 0.024 vs. 0.622 ± 0.029）, Aβ （0.430 ± 0.032 vs. 0.581 ± 0.030） expression amount were significantly lower （P〈0.05）. Conclusions Psoralen isoflavones have a certain therapeutic effect on PC12 cells injured by Aβ.