育阴软肝颗粒剂对大鼠肝纤维化的治疗作用

Therapeutic Effects of Yuyin Ruangan Granule on Liver Fibrosis and Yin-deficiency Syndrome in Rats

目的研究育阴软肝颗粒剂对大鼠肝纤维化及其阴虚证的治疗作用。方法采用多因素复制肝纤维化动物造模,通过观测大鼠肝纤维化指标(ALT、AST、肝指数、肝组织病理学、羟脯氨酸及胶原蛋白)与阴虚指标(进食与饮水量、自主活动、体质量、c AMP、c GMP),研究育阴软肝颗粒剂对实验性肝纤维化大鼠的防治作用。结果 1.08~4.32 g·kg^(-1)育阴软肝颗粒剂能降低纤维化大鼠血清ALT、AST及肝指数,降低肝组织羟脯氨酸及胶原蛋白的含量,缓解肝组织纤维化病理学变化;同时,育阴软肝颗粒剂能降低肝纤维化大鼠的饮水量,增加体质量,减少活动次数与站立次数,降低c AMP及c AMP/c GMP,缓解阴虚症状。结论多因素复制的肝纤维化大鼠模型有一定的阴虚症状;育阴软肝颗粒剂对肝纤维化及其阴虚证有一定的防治作用。

OBJECTIVE To study the therapeutic effects of Yuyin Ruangan granule（YRG） on liver fibrosis and Yin-deficiency syndrome in rats. METHODS Replicating a model of liver fibrosis through multiple factor, and researching preventive and therapeutic effect of YRG on liver fibrosis rats by measuring indexes of hepatic fibrosis（ALT, AST, liver index, liver histopathology, hydroxyproline and collagen protein） and Yin-deficiency indexes（the quantity of eating and drinking water, locomotor activity, body weight, c AMP and c GMP）. RESULTS YRG（1.08-4.32 g·kg^（-1）） could decrease ALT and AST in serum of liver fibrosis rats, reduce hepatic index and content of hydroxyproline and collagen protein, and remit liver fibrotic pathological changes. Meanwhile YRG could reduce water intake, put on weight, decrease frequency of activity and standing, and lower c AMP and c AMP/c GMP. CONCLUSION Rat model with hepatic fibrosis replicated by multi-factor has certain Yin-deficiency syndrome, and YRG has some preventive and therapeutic effects on liver fibrosis and its Yin-deficiency syndrome.