巨噬细胞移动抑制因子对肾系膜细胞增殖的影响

Effect of macrophage migration inhibitory factor on proliferation of renal mesangial cells

目的探讨MIF是否促进体外培养肾系膜细胞增殖及其可能的机制。方法体外培养大鼠肾系膜细胞(Ms C),用不同浓度的重组巨噬细胞移动抑制因子r MIF(0μg/L、50μg/L、100μg/L)刺激Ms C。CCK8法检测Ms C的增殖情况,real-time PCR及Western blot检测细胞周期蛋白D1(Cyclin D1)的mRNA和蛋白表达情况。结果 50μg/L、100μg/L的r MIF刺激Ms C 24 h及48 h后,A450显著高于对照组,差异有统计学意义(P<0.05)。r MIF刺激Ms C 48 h后,其Cyclin-D1 mRNA及蛋白表达增加。结论 MIF可能通过诱导Cyclin-D1的mRNA及蛋白表达增高,从而促进体外培养肾系膜细胞的增殖。

Objective To investigate whether MIF promotes the proliferation of mesangial cells in vitro and its possible mechanism. Methods Rat mesangial cells（ Ms C） were cultured in vitro and stimulated with different concentrations of recombinant macrophage migration inhibitory factor（ r MIF）（ 0 μg/L,50 μg/L,100 μg/L）. CCK8 was used to detect the proliferation of Ms C. Real-time PCR and Western blot were used to detect the expression of Cyclin D1 mRNA and its protein. Results When r MIF（ 50 μg/L,100 μg/L） was used to stimulate Ms C for 24 h or 48 h,A450 was significantly higher than that of control group（ P 〈 0. 05）. After r MIF stimulating Ms C for 48 h,the expression of Cyclin-D1 mRNA and protein increased. Conclusion MIF may promote the proliferation of mesangial cells in vitro by inducing the increase of mRNA and protein expression of Cyclin-D1.