紫外酶法和肌氨酸氧化酶法测定血清肌酐时对酚磺乙胺抗干扰性研究

Study on anti-interference of tanshinone by ultraviolet and sarcosine oxidase in serum creatinine

目的评价紫外酶法和肌氨酸氧化酶法测定血清肌酐时对酚磺乙胺的抗干扰性。方法参照CLSI推荐的干扰试验程序,设计干扰试验和剂量试验,判断紫外酶法和肌氨酸氧化酶法测定血清肌酐时对酚磺乙胺抗干扰性及干扰效应与酚磺乙胺浓度的关系。结果紫外酶法测定血清肌酐对酚磺乙胺具有抗干扰性,酚磺乙胺对肌氨酸氧化酶法测定血清肌酐有负干扰,肌酐浓度越低,干扰效应越大,干扰效应与酚磺乙胺浓度为二次曲线关系,酚磺乙胺对肌氨酸氧化酶法测定血清肌酐的最小干扰浓度为0.009 g/L。约经过5个半衰期(大约10 h)血中酚磺乙胺浓度降到0.009 g/L左右。结论紫外酶法测定血清肌酐对酚磺乙胺具有抗干扰性,对结果无影响,推荐各实验室使用该方法;酚磺乙胺对肌氨酸氧化酶法测定血清肌酐有负干扰,用药后大约10 h对结果无影响。

Objective To evaluate the anti-interference of phenylethanolamine when ultraviolet spectrophotometry and sarcosine oxidase were used to measure serum creatinine. Methods According to the interference test program recommended by CLSI,the interference test and the dose test were designed to determine the anti-interference effect of phenylethylamine on serum creatinine by ultraviolet enzyme and sarcosine oxidase,and to explore the relationship between interference effect and the concentration. Results Ultraviolet enzymatic determination of serum creatinine had an anti-interference effect to metsulfuron-methyl. Ethanesulfonamide had negative interference with creatinine oxidase method in the determination of serum creatinine.The lower the creatinine concentration was,the greater the interference effect was. The concentration of amines was a quadratic curve,and the minimum interference concentration of phenylethylamine for creatinine was 0. 009 g/L. Approximately 5-fold half-life（ about 10 h） of phenylethylamine sulfonate concentration dropped to about 0. 009 g/L. Conclusion UV enzymatic determination of serum creatinine has anti-interference to the phenylethanolamine,no effect on the results,so this method is recommended for laboratory use; phenol sulfonamide has negative interference on creatine oxidase method for the determination of serum creatinine,and after treatment about 10 h,it has no effect on the result.