摘要
由多主棒孢(Corynespora cassiicola)侵染引起的黄瓜棒孢叶斑病已成为我国黄瓜生产上的重要新流行病害,目前尚缺乏抗性品种,病原菌侵染机制及与寄主的互作关系尚不清楚。为了开展多主棒孢的病原学研究,本试验采用农杆菌基因介导技术(ATMT),获得了红色荧光蛋白(RFP)标记的多主棒孢转化株,转化株在PDA培养基转接6次后仍能在菌丝和分生孢子上发出强烈的红色荧光,生物学测定和致病性测定结果表明该转化株与野生型菌株无显著差异。
Cucumber Corynespora leaf spot caused by Corynespora cassiicola has become an important new epidemic disease in cucumber production in China.At present,there is no resistant varieties and the infection mechanism of C. cassiicola and its interaction with host are still unknown.In order to investigate the pathogen of C. cassiicola,the RFP-labeled transformant was generated with Agrobacterium tumefaciens-mediated transformation(ATMT).The transformant was able to emit strong red fluorescence on mycelia and conidia during 6 passages of transformants on PDA.The biological determination and pathogenicity test demonstrated that there was no significant differences between the wild strain and the transformant.
出处
《中国蔬菜》
北大核心
2018年第3期45-49,共5页
China Vegetables
基金
国家自然科学基金项目(31401888)
国家大宗蔬菜产业技术体系项目(CARS-25)
关键词
黄瓜棒孢叶斑病
多主棒孢
农杆菌介导法
RFP
Cucumber Corynespora leaf spot
Corynespora cassiicola
Agrobacterium mediated
RFP
