咽炎消合剂对慢性咽炎肝郁脾虚大鼠5-HT调控机制研究

Study on the regulation mechanism of Yanyanxiao mixture on 5-HT in rats with chronic pharyngitis of liver depression and spleen deficiency type

目的观察咽炎消合剂对慢性咽炎大鼠血清5-羟色胺(5-HT)水平的影响,探讨慢性咽炎的发生发展与影响情绪的关键神经递质5-HT异常表达的相关性。方法将49只Wistar大鼠随机分为空白对照组、标准模型组、标准模型药物低剂量组、标准模型药物高剂量组、肝郁脾虚模型组、肝郁脾虚药物低剂量组、肝郁脾虚药物高剂量组,每组7只。标准模型各组构建慢性咽炎标准模型,肝郁脾虚模型各组构建慢性咽炎肝郁脾虚型模型,造模成功后各药物组分别给予相应剂量咽炎消合剂灌胃,空白对照组和模型组给予生理盐水灌胃。灌胃10 d后检测各组大鼠血清5-HT水平,取咽部组织进行HE染色观察。结果标准模型组和肝郁脾虚模型组血清5-HT水平均明显低于空白对照组(P均<0.05),标准模型组和肝郁脾虚模型组比较差异无统计学意义(P>0.05);标准模型给药组和肝郁脾虚给药组血清5-HT水平均明显高于相应模型组(P均<0.05),且肝郁脾虚给药组血清5-HT水平明显高于标准模型给药组(P均<0.05)。标准模型和肝郁脾虚模型各给药组大鼠咽黏膜慢性炎细胞数量均明显减少,咽部血管通透性及炎细胞渗出减轻。结论慢性咽炎与5-HT的代谢异常具有一定的相关性,咽炎消合剂除了能够控制和消除局部炎症症状外,对神经中枢也具有调节作用。

Objective It is to observe the effect of Yanyanxiao mixture on serum 5-HT level in chronic pharyngitis rats of liver depression and spleen deficiency type,and explore the relationship between the occurrence and development of chronic pharyngitis and the abnormal expression of neurotransmitter 5-HT. Methods Forty-nine Wistar rats were randomly divided into 7 groups： control（ C） group,normal model（ NM） group,NM-lowdose（ NM-LD） group,NM-highdose（ NMHD） group,liver depression and spleen deficiency model（ LDSDM） group; LDSDM-lowdose（ LDSDM-LD） group,and LDSDM-highdose（ LDSDM-HD） group,7 cases in each group. The standard model of chronic pharyngitis or the liver depression and spleen deficiency model of chronic pharyngitis were constructed respectively. After the success of the model,the drug groups were given the corresponding dose of pharyngitis mixture,and normal saline was given to the C group and the NM group. The serum 5-HT level of rats in each group was detected after gavaged for 10 days. The tissue of the pharynx was observed by HE staining. Results The levels of serum 5-HT in the NM group and the LDSDM group were significantly lower than that of the C group（ all P 0. 05）,there was no significant difference between C group and NM group（ P 0. 05）. The concentrations of 5-HT in NM drug group and LDSDM drug group were significantly higher than the corresponding model group（ all P 0. 05）,and the levels of the LDSDM drug groups were significantly higher than those of the NM drug group（ all P 0. 05）. The number of chronic inflammatory cells in the pharyngeal mucosa of the two type of models was significantly reduced,the pharyngeal vascular permeability and the inflammatory cell exudation were reduced too.Conclusion 5-HT metabolic abnormalities has a certain correlation with chronic pharyngitis. Yanyanxiao mixture can control and eliminate local inflammatory symptoms,and has a regulatory role on nerve central system.