超高效液相色谱-串联质谱法同时测定抗鼻炎中成药及保健品中19种化学药物

Simultaneous Determination of 19 Chemical Drugs in Traditional Chinese Medicines and Health Products for Treating Rhinitis by UPLC-MS/MS

目的建立同时测定抗鼻炎类中成药及保健品中添加的19种化学药物的超高效液相色谱-串联质谱分析方法。方法采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以0.1%甲酸乙腈溶液-0.1%甲酸溶液为流动相,梯度洗脱,流速0.3 m L·min-1,柱温35℃,选择电喷雾电离源正离子扫描(ESI+),多反应监测模式(MRM)进行检测。结果 19种化学药物在各自质量浓度范围内线性关系较好,相关系数均>0.995,低、中、高浓度水平平均回收率84.9%~111.1%,RSD均<5.2%,检测限0.03~0.78 ng·m L-1,定量限0.13~1.82 ng·m L-1。结论该方法简便、快速、准确,灵敏度高,可作为抗鼻炎类中成药及保健品中非法添加化学药物的检测方法。

Objective To establish a UPLC-MS/MS method for simultaneous determination of 19 chemical drugs in Traditional Chinese Medicines and health products for treating rhinitis. Methods Separation was performed on Waters ACQUITY UPLC BEH-C18 column( 2.1 mm×100 mm,1.7 μm) with 0.1% formic acid acetonitrile solution and 0.1% formic acid aqueous solution as the mobile phase by gradient elution.The flow rate was 0.3 m L·min-1.The column temperature was set at 35 ℃.The detection was performed by the positive ion electrospray ionization( ESI+) under multiple reactions monitoring( MRM)mode. Results The linear relationships of 19 chemical drugs were good in respective ranges with correlation coefficients higher than 0.995.The average recoveries of the low,medium,and high level were in the range of 84.9%-111.1%,and the RSDs were less than 5.2%.The limits of detection( LOD) and the limits of quantitation( LOQ) were in the range of 0.03-0.78 ng·m L-1 and 0.13-1.82 ng·m L-1,respectively. Conclusion The method is convenient,rapid,accurate,and sensitive,which can be used for the determination of chemical drugs added illegally in traditional Chinese medicines and health products for treating rhinitis.