我国传统干酪中生物胺含量检测分析

Determination and analysis of biogenic amines in Chinese traditional cheese

通过反相高效液相色谱法对来自内蒙古、新疆、西藏、云南、北京市场共17种中国传统干酪样品生物胺含量进行了分析。结果表明,反相高效液相色谱法能准确地测定干酪中生物胺含量;在全部干酪样品中,8种生物胺含量大小不一,生物胺总量在1.30~41.27 mg/kg范围内,均低于国家标准规定要求,其中有14个样品的生物胺总量低于10 mg/kg,第10号干酪生物胺含量为41.27 mg/kg,为所有样品中含量最高,其次为第1号样品和第9号样品,分别为18.48 mg/kg和10.35 mg/kg。相关性分析结果显示,色胺与苯乙胺,腐胺与尸胺、酪胺、亚精胺,组胺与精胺,酪胺与精胺之间在0.01水平上具有显著相关性。

The contents of biogenic amines in 17 Chinese traditional cheese samples which were collected i^om 5 different regions including Inner Mongolia, Xinjiang, Tibet, Yunnan and Beijing were analyzed using reverse-phase high performance liquid chromatography （RP-HPLC）. The results showed that RP-HPLC method could accurately determine the contents of biogenic amines in cheese. In all cheese samples, the contents of 8 kinds of biogenic amines varied in size and the total contents were in the range of 1.30-41.27 mg/kg, which was lower than the national standard requirements. The total contents of biogenic amines in 14 samples were less than 10 mg/kg. The content of biogenic amine in sample No.10 was 41.27 mg/kg, which was the highest in all samples, followed by sample No.1 and sample No.9, which were 18.46 mg/kg and 10.35 mg/kg, respectively. The results of correlation analysis showed that there was a significant correlation at 0.01 level between tryptamine and phenethylamine, putrescine and cadaver- ine, tyramine, spermidine, as well as histamine and spermine, tyramine and spermine.