辣椒类甜蛋白基因家族鉴定及表达分析

Identification and expression analysis of thaumatin-like protein gene in pepper

为了在全基因组水平上了解辣椒类甜蛋白基因(TLP)家族特征,本研究通过生物信息学方法,利用辣椒基因组数据库,对筛选的28个辣椒TLP家族成员进行鉴定,对结构功能、聚类及时空表达进行分析。结果表明,辣椒TLP家族基因主要分为4种结构类型,基因分布在9条染色体上。系统发育分析结果显示,辣椒TLP家族基因属于8个聚类组,其中成员最多的聚类组5中的基因主要来自1号染色体。基因启动子区顺式作用元件分析发现多个激素响应元件、生物/非生物响应元件。时空表达分析结果表明,多数辣椒TLP家族基因在各器官和果实发育的各阶段均有较高表达,各成员在时空表达上具有器官特异性。本研究为辣椒TLP家族基因的克隆及其在植物生长及对抗胁迫方面的研究奠定了基础。

To understand the characteristics of pepper thaumatin-like protein( TLP) gene family on the genomic level,gene structure and phylogenesis of 28 pepper TLP family members were analyzed and identified through pepper genome database and bioinformatics methods. The results showed that the pepper TLP gene structure was classified into four types,and TLP genes distributed on nine of twelve chromosomes. Phylogenetic analysis showed that the pepper TLP family genes were classified into eight evolutionary groups. Most members of group five mainly derived from the first chromosome. The spatiotemporal expression analysis showed that most of the TLP family genes were highly expressed in various organs and fruit development stages. Each member had organ specificity in temporal and spatial expression. This study laid a foundation for cloning of pepper TLP genes and further functional dissection in organ development.