外源腐胺和D-精氨酸对低温胁迫下红掌生理生化指标的影响

Effects of exogenous putrescine and D-Arg on physiological and biochemical indices of anthurium under chilling stress

为研究外源腐胺(Put)对红掌冬季生长过程中低温胁迫的缓解作用,以红掌品种阿拉巴马为试验材料,在6℃低温胁迫下,探讨不同浓度(0.5 mmol/L、1.0 mmol/L、1.5 mmol/L、2.0 mmol/L)的Put和1.0 mmol/L D-精氨酸(D-Arg)对红掌生理生化指标的影响。结果表明:根外施用一定浓度的Put后,红掌根系的抗氧化酶活性、根系活力、叶片氮代谢水平、叶绿素含量、脯氨酸(Pro)含量明显提高,丙二醛(MDA)含量降低,且以1.0 mmol/L Put处理效果最佳,D-Arg处理加重了低温对红掌的伤害。说明Put参与了红掌的抗冷调节,根外施用一定浓度的Put能缓解低温胁迫对红掌生长的不利影响。

The alleviation of chilling stress by putrescine( Put) were studied on Anthunium andraenum Alabamb subjected to different concentrations( 0. 5 mmol/L,1. 0 mmol/L,1. 5 mmol/L,2. 0 mmol/L) of Put and 1. 0 mmol/L of D-Arg under 6 ℃ temperature. Physiological and biochemical indices were explored to indicate the alleviation. The antioxidant enzymes activities,root activity,level of nitrogen metabolism,chlorophyll content and proline( Pro) content of Anthurium andraeanum were significantly increased,but content of malondialdehyde( MDA) decreased. The treatment of 1. 0 mmol/L Put showed the best effect. The chilling injury was deteriorated in the treatment of D-Arg. In conclusion,Put took part in the cold resistance regulation,and the adverse effects of chilling stress on the growth of Anthurium andraeanum were alleviated by using proper amount of Put.