2013-2015年深圳市福田区人星状病毒感染流行病学及分子特征分析

Epidemiological and genetic characteristics of human astrovirus in Futian district, Shenzhen, 2013-2015

目的 研究深圳市福田区腹泻患者人星状病毒（human astrovirus,HAstV）感染的流行病学及分子特征.方法 收集2013年1月至2015年12月深圳市福田区1 625例疑似腹泻患者粪便标本,采用实时荧光定量逆转录-聚合酶链反应（RT-qPCR）方法检测粪便中的HAstV核酸,RT-qPCR阳性样品使用Mon269/Mon270引物对,用荧光逆转录-聚合酶链反应（RT-PCR）扩增HAstV的开放阅读框ORF2区域,将RT-PCR扩增产物纯化、测序后进行序列分析,并分析病例的流行病学资料.结果 2013-2015年福田区HAstV的检出率为2.03％（33/1 625）,其中2013年的检出率为1.84％（12/653）,2014年的检出率为1.31％（7/535）,2015年的检出率为3.20％（14/437）;男性检出率为2.27％（21/925）,女性检出率为1.71％（12/700）,男女患者检出率的差异无统计学意义（x2=0.62,P=0.431）.HAstV在各年龄组人群均有检出,但在1岁以下年龄组患者的检出率最低,为1.69％（11/650）.HAstV在1月份的检出率最高（5.59％,8/143）,7月份的检出率最低（未检出）.将33份RT-qPCR检测为HAstV阳性的样品用开放阅读框ORF2区进行分型,结果有27份可进一步分型,其中HAstV-1检出15株,是福田区HAstV的主要流行株,此外分别检出7株HAstV-4、4株HAstV-5和1株HAstV-2.结论 HAstV是导致福田区病毒性腹泻的病原体,HAstV-1是福田区HAstV的主要流行株,同时也存在其他型别的感染.

Objective To investigate the epidemiological and genetic characteristics of human astorvirus （HAstV） infections in Futian district,Shenzhen.Methods A total of 1 625 stool samples were collected from patients with suspected diarrhea in Futian district,Shenzhen from January 2013 to December 2015.The HAstV RNA was detected by reverse transcription quantitative polymerase chain reaction （RT-qPCR）,and the ORF2 of HAstV in positive samples was amplified and sequenced by RT-PCR with primer set of Mon269/Mon270,followed by phylogenetic analysis.Results From January 2013 to December 2015,the overall detection rate of HAstV was 2.03% （33/1 625）,while the annual detection rates were 1.84% （12/653）,1.31% （7/535） and 3.20% （14/437）,respectively.The detection rate of HAstV was 2.27% （21/925） among males and 1.71% （12/700） among females.There was no significant difference in the detection rate between male and female （x2=0.62,P=-0.431）.HAstV was detected in all age groups,while the detection rate was significantly lower in age group 〈 1 year old （1.69%,11/650）.The detection rate was the highest in January （5.59%,8/143）,and was the lowest in July （no case was detected）.Thirty-three positive samples were sequenced and analyzed,HAstV-1,HAstV-4,HAstV-5 and HAstV-2 were detected in 15,7,4,1 sample（s）,respectively,of which HAstV-1 was the predominant strain.Conclusions HAstV was a pathogen causing viral diarrhea in Futian district.HAstV-1 was the predominant strain,while other genotypes also existed.