氨基化Fe_3O_4纳米粒子的制备及对胃癌细胞的毒性评价

Preparation of Aminosilane-modified Fe_3O_4 Nanoparticles and Its Cytotoxicity Evaluation on Gastric Cancer Cells

采用共沉淀法制备了四氧化三铁纳米粒子(Fe_3O_4),并在Fe_3O_4纳米粒子表面修饰上了氨基。透射电子显微镜(TEM)和傅里叶变换红外光谱(FTIR)显示,纳米粒子分散性良好,粒径约为10 nm,氨基成功修饰在了纳米粒子的表面。以胃癌SGC-7901为目的细胞,依据RGR值考察了Fe_3O_4-NH_2纳米粒子的细胞毒性等级,结果显示,在一定浓度范围内,细胞死亡率与Fe_3O_4-NH_2纳米粒子的浓度成正相关,低于51.2μg/m L时,Fe_3O_4-NH_2纳米粒子的细胞毒性相对较小,Fe_3O_4-NH_2纳米颗粒具有较好的生物相容性。

Fe_3O_4 nanoparticles were prepared by the chemical co-precipitation method,and the aminosilane-modified nanoparticles was modified with 3-aminopropyltriethoxysilane( Fe_3O_4-NH_2). The aminosilane-modified nanoparticles were analyzed by TEM and FTIR. The results showed that nanoparticles had a good dispersion and the particle size was about 10 nm. The amino groups were successfully modified on the surface of the Fe_3O_4 nanoparticles. The cytotoxicity evaluation of Fe_3O_4-NH_2 nanoparticles on gastric cancer( SGC-7901) cells were investigated based on RGR values. Results showed that cell mortality was closely relative to the concentration of the nanoparticles in the certain concentration range. The cytotoxicity of nanoparticles was relatively small when the concentration was below 51. 2 μg/m L.Fe_3O_4-NH_2 nanoparticles had good biocompatibility.