HBP、IL-17A、IL-17F等炎性因子在小儿脓毒症早期诊断中的价值对比研究

Comparative research of inflame factors such as HBP,IL-17A,IL-17F in child patients with sepsis

目的比较脓毒症前不同时间点肝素结合蛋白等炎性因子的诊断效能。方法选取2014年1月-2016年10月本院小儿脓毒症患者24例,收集其诊断脓毒症的时刻0 h、前24 h、前48 h、前72 h血浆,同期选取22例无器质性疾病的健康体检者抽取血浆作为对照组,酶联免疫吸附法检测HBP、IL-17A、IL-17F。结果 HBP在前48 h、24 h、0 h时间点均高于其前一时间点水平(P<0.01),所有时间点均高于对照组(P<0.01)。受试者工作特征曲线(ROC曲线)显示,HBP在前72 h、前48 h、前24 h、0 h的AUC分别为0.823、0.898、0.935、1.000,但只有前72 h明显高于其他2种细胞因子。结论 HBP具有较宽的诊断窗口期,适合作为小儿脓毒症的早期预测指标。

Objective To compare the diagnostic efficacy of inflammatory factors such as heparin-binding protein（ HBP） at different time points before the incidence of sepsis. Methods 24 child patients with sepsis from January 2014 to October 2016 were selected. Blood samples were collected at 0 h,24 h,48 h,72 h before the diagnosis of sepsis,and 22 healthy volunteers without organic disease were selected as the control group and their plasma was taken during the same period. Enzyme-linked immunosorbent assay was used to detect HBP,IL-17 A,IL-17 F. Results The serum levels of HBP at all time points were all higher than that at the former point,and significantly higher than the control group（ P〈 0. 01）. Receiver operating characteristic curve（ ROC） revealed that the areas at 72 h,48 h,24 h,0 h before the diagnosis time were 0. 823,0. 898,0. 935,1. 000,but only that at 72 h was higher than the other 2 interleukins. Conclusion HBP has a wide diagnostic window period for sepsis,which is suitable as an early predictor of pediatric sepsis.