妊娠性血小板减少症患者与正常孕妇血小板膜糖蛋白2b/3a及淋巴细胞亚群水平的差异

Differences of levels of platelet membrane glycoprotein 2b/3a and lymphocyte subgroups between patients with pregnancy-associated thrombocytopenia and normal pregnant women

目的研究妊娠性血小板减少症(GT)患者与正常孕妇血小板膜糖蛋白2b/3a浓度和淋巴细胞亚群之间的差异性,为GT在临床中的鉴别诊断提供依据。方法选取2014年8月-2016年9月于该院确诊妊娠、妊娠前血小板检测无异常且在妊娠期间未出现除GT外的其他合并症或并发症的孕妇共47例。采用流式细胞术检测孕妇淋巴细胞亚群和Th1、Tc1、Th17细胞占CD3+T细胞比例;采用酶联免疫吸附测定法(ELISA)测定其血浆IL-17、干扰素-γ(IFN-γ)因子水平;采用改良的单克隆抗体特异性俘获血小板抗原技术(MAIPA)测定血小板膜糖蛋白2b/3a水平。分娩结束后,根据血小板检测结果,分为GT组(33例)和对照组(14例),对比两组数据。结果 GT组血小板压积(0.08±0.02)%显著低于对照组的(0.18±0.04)%,血小板计数(68.01±14.69)×10~9/L显著低于对照组的(169.48±49.32)×10~9/L,差异有统计学意义(P<0.05);GT组IL-17因子水平为(14.26±14.18)pg/ml,与对照组的(15.11±15.06)pg/ml比较,差异无统计学意义(P>0.05);GT组IFN-γ因子水平为(24.29±19.34)pg/ml,与对照组的(23.14±18.19)pg/ml比较,差异无统计学意义(P>0.05);GT组的血小板膜糖蛋白2b/3a OD值对比,差异无统计学意义(P>0.05)。结论 GT患者和正常孕妇血小板膜糖蛋白2b/3a浓度和淋巴细胞亚群之间对比无明显差异。

Objective To study the differences of levels of platelet membrane glycoprotein 2 b/3 a and lymphocyte subgroups between patients with pregnancy-associated thrombocytopenia and normal pregnant women,and provide a basis for clinical identification and diagnosis of pregnancy-associated thrombocytopenia. Methods Forty-seven pregnant women with normal platelet detection result and without other complications except pregnancy-associated thrombocytopenia were selected from People＇s Hospital of Kaizhou District from August 2014 to September 2016. Flow cytometry was used to detect lymphocyte subgroups and proportions of Th1,Tc1,and Th17 cells in CD3＋T cells.ELISA was used to detect the levels of plasma IL-17 and interferon-γ（ IFN-γ）. Improved MAIPA was used to detect the level of platelet membrane glycoprotein 2 b/3 a. After delivery,the women were divided into study group（ 33 women with pregnancy-associated thrombocytopenia） and control group（ 14 women without pregnancy-associated thrombocytopenia） according to the detection results of platelet.Results Thrombocytocrit in study group was（ 0. 08 ± 0. 02） %,which was statistically significantly lower than that in control group[（ 0. 18±0. 04） % ]（ P〈0. 05）. Platelet count in study group was（ 68. 01±14. 69） ×10~9/L,which was statistically significantly lower than that in control group [（ 169. 48±49. 32） ×10~9/L]（ P〈0. 05）. The levels of IL-17 in study group and control group were（ 14. 26±14. 18） pg/ml and（ 15. 11±15. 06） pg/ml,respectively,there was no statistically significant difference between the two groups（ P〈0. 05）.The levels of IFN-γ in study group and control group were（ 24. 29±19. 34） pg/ml and（ 23. 14±18. 19） pg/ml,respectively,there was no statistically significant difference between the two groups（ P〈0. 05）. In study group,there was no statistically significant difference in OD value of platelet membrane glycoprotein 2 b/3 a. Conclusion There is no significant difference in platelet membrane glycoprotein 2 b/3 a and lymphocyte subgroups between patients with pregnancy-associated thrombocytopenia and normal pregnant women.