云南省食源性金黄色葡萄球菌脉冲场凝胶电泳分型分析

Molecular typing of foodborne Staphylococcus aureus by pulsed-field gel electrophoresis in Yunnan province

目的采用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)方法分析云南省2013～2015年食源性金黄色葡萄球菌分子分型带型,初步建立金黄色葡萄球菌PFGE分型的数据库。方法用限制性内切酶Smal I酶切113株金黄色葡萄球菌染色体DNA以进行PFGE分析,并利用BioNumerics软件对分离株的指纹图谱进行聚类分析。结果 113株食源性金黄色葡萄球菌的PFGE图谱的相似性系数在56.67%～100%之间,经聚类分析得到89个PFGE型别。结论建立云南省食源性金黄色葡萄球菌PFGE分型数据库,PFGE带型呈现多样性,对今后云南省金黄色葡萄球菌引起食物中毒的诊断和溯源工作有重要意义。

Objective To analyze molecular genotype of foodborne Staphylococcus aureus by pulsed-field gel electrophoresis（PFGE） and establish the PFGE typing database in Yunnan province during 2013 to 2015. Methods A total of 113 kinds of foodborne strains were digested with restriction endonucleases Smal I and analyzed by PFGE. The fingerprints of the isolates were clustered by Bio Numerics software. Results Totally 89 PFGE types were obtained among the 113 strains through cluster analysis. The similarity coefficients of the PFGE atlas were in the range of 56.67%～100%. Conclusion Diversity PFGE database of foodborne Staphylococcus aureus is established in Yunnan province, which is of great significance for diagnosis and derivation of the food-poisoning causing by Staphylococcus aureus.