猪源性动物产品中猪链球菌2型分离方法的建立和应用

Establishment and application of isolation methods for Streptococcus suis type 2 in pig-derived products

目的建立猪源性动物产品中猪链球菌2型分离方法,为监测人畜共患致病菌猪链球菌2型提供技术支持。方法通过选择不同增菌基础液、不同种类和不同浓度的抑菌剂及不同选择性分离平板,建立猪源性动物产品中猪链球菌2型的分离方法。对从920份猪源性动物产品分离到的可疑菌落进行初步筛选、生化鉴定、血清学鉴定和分子鉴定。结果使用该分离方法检测猪源性动物产品,背景细菌显著减少,猪链球菌典型菌落显著增加,分离更加容易。从920份猪源性动物产品中共检出27株猪链球菌,阳性率为2.93%(27/920),其中猪链球菌2型为17株,阳性率为1.85%(17/920)。结论本方法为猪源性动物产品中猪链球菌2型检测提供了技术手段。

Objective To establish isolation methods for detection of Streptococcus suis type 2, so as to provide technical support for monitoring zoonotic pathogen in pig-derived products. Methods By choosing different enrichment base fluid, different concentrations of different kinds of bacteriostatic agent and different selective separating plates, a method for isolation of Streptococcus suis serotype 2 in pig-derived animal products was established. Preliminary screening, biochemical identification, serological identification and molecular identification of suspicious colonies isolated from 920 pig-derived products were carried out. Results It was easier to isolate Streptococcus suis type 2 in pig-derived products, where the background bacteria were significantly reduced, and the typical colonies increased significantly. A total of 27 strains of Streptococcus suis were detected from 920 pig-derived products, with the positive rate of 2.93%（27/920）, and 17 strains were Streptococcus suis type 2, with positive rate of 1.85%（17/920）. Conclusion The established method is suitable for detection of Streptococcus suis type 2 in pig-derived products.