进口肉类中恶臭假单胞菌的分离与鉴定

Isolation and identification of Pseudomonas putida from imported meat

目的选择具有较高选择性的培养基,分离与鉴定进口肉类中恶臭假单胞菌。方法参照GB4789.4-2010《食品安全国家标准食品微生物学沙门氏菌检验》、SN/T2099-2008《进出口食品中绿脓杆菌检测方法》,根据VITEK2显示的结果做生化鉴定。结果在选择性较高的沙门氏菌显色培养基上生长的菌落形态与沙门氏菌极为相似,VITEK2显示结果为恶臭假单胞菌,后经生化鉴定,结果与VITEK2一致,为恶臭假单胞菌。结论沙门氏菌显色培养基难以区分假单胞菌,但是亚硫酸铋琼脂(bismuth agar,BS)却可以明显区分,所以在日常检测过程中建议不要省去BS平板,在配制BS平板时需严格按照说明进行,以保证实验效果。

Objective To choose a medium with higher selectivity, and isolate and identify the Pseudomonas putida from imported meat. Methods According to GB 4789.4-2010 National food safety standard-Food microbiological examination： Salmonella and SN/T2099-2008 Determination of Pseudomonas aeruginosa in food for import and export, the biochemical identification was based on the results shown in VITEK2. Results The fungus which grew on the Salmonella culture medium was very similar to Salmonella in the detection process, but the identification result was Pseudomonas putida by VITEK2, and biochemical identification showed that the result was consistent with VITEK2, which was Pseudomonas putida. Conclusion Salmonella chromogenic medium is difficult to differentiate Pseudomonas, but sulfurous acid bismuth agar（BS） can clearly distinguish, so eliminating BS tablet is not recommended in daily inspection, and the preparation of BS tablet should strictly follow the instructions, to ensure the experimental results.