3种B群链球菌显色培养基性能比较研究

Comparative research on the performance of 3 different kinds of group B Streptococcus chromogenic media

目的通过比较3种B群链球菌显色培养基的性能,了解国内外B群链球菌显色培养基的技术现状。方法用8株菌株和212例临床白带标本分别接种国内外3种B群链球菌显色平板或培养基制作的平板,比较目的菌生长和显色差异、杂菌被抑制和显色的差异以及临床标本的检出情况。结果 3种B群链球菌显色培养基灵敏度一致,均为92.30%(12/13),国产M1公司、进口M2公司以及进口M3公司B群链球菌显色培养基的特异性分别是98.99%(197/199)、98.49%(196/199)和96.98%(193/199),但阳性预期值差异较大,分别是85.71%(12/14)、80.00%(12/15)和66.67%(12/18),M1公司产品与M2公司产品结果差异不显著(P>0.05),但M3公司产品与二者均差异显著(P<0.05)。结论国产B群链球菌显色培养基的整体性能明显优于进口产品,有较大的临床应用价值。

Objective To evaluate the 3 kinds of group B Streptococcus chromogenic media, and assess the technical status of them made in domestic and overseas. Methods A total of 8 strains and 212 cases of clinical vaginal secreta were cultured on the medium made by 3 different manufacturers, respectively. The differences of the growth and chromogenic reaction of the target bacteria as well as inhibition of the non-target bacteria and the performance of detection of clinical specimens were compared. Results The sensitivities of the 3 kinds of media were comparable with of 92.30%（12/13）. The specificities of the 3 kinds of chromogenic media made by domestic company M1, foreign company M2 and foreign company M3 were 98.99%（197/199）, 98.49%（196/199） and 96.98%（193/199）, respectively. There was much more significant difference between the positive predictive values of them, which were 85.71%（12/14）, 80.00%（12/15） and 66.67%（12/18）, respectively. There was no statistically significant difference between the products made by company M1 and company M2（P〉0.05）, but the difference between the products made by company M3 and the other two companies was statistically significant（P〈0.05）. Conclusions The overall performance of domestic group B Streptococcus chromogenic medium is better than imported products, which has a greater value of clinical application.