3种口蹄疫O型抗体检测试剂盒对疫苗免疫牛血清抗体检测的比较试验

Comparison of three ELISA kits for detection of cattle foot-and-mouth disease virus type O antibody

为了提供检测结果准确且操作便捷的试剂盒,本试验应用两种商品化试剂盒和本实验室研制的一种基于合成肽包被抗原的间接ELISA试剂盒同时对口蹄疫疫苗免疫后14、21和28 d的150份免疫牛血清进行口蹄疫O型抗体水平的检测,并对3种试剂盒测定结果的符合程度、重复性和便捷性进行了比较。试验结果表明3种试剂盒测得的抗体水平检测结果之间高度符合,且3种试剂盒各自的检测结果重复性良好,基于合成肽包被抗原的间接ELISA试剂盒在操作便捷性方面优势明显。

In order to evaluate the accuracy, reproducibility and convenience of an indirect ELISA kit based on synthetic peptidecoated antigen, and provide accurate and convenient kit for clinical use, two commercial kits and an indirect ELISA kit based on syn-thetic peptide coated antigen developed by our laboratory were used in this study. At 14, 21 and 28 days after immunization, 150 se-rum samples were collected and detected with three kits. The coincidence degree, repeatability and convenience between the threekits are compared. The results showed that there was a good agreement between three kits. The results of the three kits were repro-ducible, and the kit developed by us has obvious advantages over convenient operation.