甲烷饱和盐水对单关节炎大鼠镇痛作用的研究

Analgesic effect of methane rich saline in monoarthritis model rat

目的 探讨甲烷饱和盐水（MS）对于单关节炎大鼠镇痛作用及其可能机制.方法 利用完全弗氏佐剂（CFA）诱导单关节炎（MA）大鼠模型,并通过痛行为学评估、蛋白印迹、酶联免疫吸附分析和氧化应激检测观察MS对MA大鼠的影响.结果 腹腔内注射MS可以缓解MA大鼠的机械触模痛,抑制脊髓背角胶质细胞的活化和炎性因子的表达,也可以降低脊髓背角的氧化应激的水平.结论 甲烷饱和盐水对MA大鼠具有镇痛作用,且这一作用可能是通过抗神经炎症及抗氧化作用来实现的.

Objective To investigate the analgesic effect of methane rich saline （MS） on monoarthritis （MA） rat and its possible mechanism.Methods We utilized Complete Freund＇s Adjuvant （CFA） to induce MApain model,and observed the effect of MS on MA rats by behavior assess,western blot,enzyme linked immunosorbent assay（ELISA）,and oxidative stress assay.Results Intraperitoneal injections of MS attenuated MA-induced mechanical allodynia.MS treatments inhibited the activation of microglia and astrocytes,decreased IL-1β and TNF-α production in the spinal cord on day 10 post-operation.Additionally,MS also suppressed MA-induced oxidative stress （MDA）,and protected the antioxidant capacity （SOD and CAT） in spinal cord dorsal horn.Conclusions Our findssuggested the analgesic effect of MS on monoarthritis pain,and imply antineuroinflammation and anti-oxidative stress as the associated mechanisms.