2016年河南省市售生禽肉中沙门菌血清分型和分子分型研究

Characteristics of serotyping and molecular typing of Salmonella isolated from raw poultry in Henan 2016

目的了解河南省市售生禽肉中沙门菌污染状况,并对分离株进行血清型和分子分型研究,为河南省食源性疾病溯源数据库提供基础数据。方法沙门菌检测及血清分型、脉冲场凝胶电泳(PFGE)分子分型分析参照国家食品及食源性疾病监测网工作手册。结果 165份生禽肉样品中检出41株沙门菌,分属13个血清型,优势血清型是科瓦利斯沙门菌、肯塔基沙门菌、鼠伤寒沙门菌及达布沙门菌。经Xba Ⅰ酶切,获得30种带型,每种带型包括1~5株菌株,相似度为47.5%~100%。部分不同血清型沙门菌PFGE型相似。结论河南省市售生禽肉沙门菌污染严重,沙门菌血清型和基因型呈现多样化,基因型有一定的地区聚集性。

Objective To learn the status of Salmonella contamination in raw poultry sold in Henan,the serotypes and genotypes of the positive isolates were studied to provide basic data for foodborne disease traceability database in Henan.Methods Salmonella detection,serotyping and pulsed field gel electrophoresis（ PFGE） molecular typing were performed by the manual for food and foodborne diseases monitoring network. Results Forty-one strains of Salmonella were detected from 165 samples and the detection rate was 24. 85 %（ 41/165）. The 41 strains of Salmonella had 13 serotypes,and the dominant serotypes were Salmonella corvallis,Salmonella kentucky,Salmonella typhimurium and Salmonella dabou. Fortyone strains were divided 30 molecular patterns by Xba I digestion and pulsed field gel electrophoresis,and each pattern contained 1-5 strains with similarity ranged from 47. 5 %-100 %. The PFGE patterns were similar in different serotypes.Conclusion The contamination of Salmonella in raw poultry was serious in Henan, the serotypes and genotypes of Salmonella were diversified,and genotypes had certain regional aggregation.