初诊2型糖尿病患者循环miRNA-101的表达变化及临床意义

Clinical implications of circulating miRNA-101 expression level in patients with newly diagnosed type 2 diabetes mellitus

目的:测定初诊2型糖尿病(type 2 diabetes mellitus,T2DM)患者血清miRNA-101的表达水平,分析其变化的临床意义。方法:采用qRTPCR法检测30例初诊T2DM患者及正常受试者血清中miRNA-101表达水平,并测定相关临床指标。两因素间关系采用Pearson相关分析。评估miRNA-101和其他参数的关联应用多元逐步回归分析。结果:初诊T2DM患者血清中miRNA-101表达水平显著高于正常对照组(P<0.05)。血清miRNA-101表达水平与空腹血糖(fasting plasma glucose,FPG)和糖化血红蛋白A1c(glycosylated hemoglobin A1c,HbA1c)呈正相关(P<0.05)。在校正性别、年龄、体质量后,多元逐步回归分析表明miRNA-101水平与HbA1c呈显著正相关(P<0.05)。结论:初诊T2DM患者血清miRNA-101表达水平增高,可能与HbA1c升高有关。

AIM： To investigate the serum miRNA-101 expression level in patients with newly diagnosed type 2 diabetes mellitus（ T2DM）,and to evaluate the clinical implications of miRNA-101 expression level variation.METHODS： qRT-PCR was used to determine the serum miRNA-101 expression level.Pearson correlation analysis was performed to observe the relationship between two variables.Multiple stepwise linear regression analysis was used to assess the association of serum miRNA-101 level and other parameters.RESULTS： Serum miRNA-101 level in patients with newly diagnosed T2DM was significantly higher than that in control subjects（ P〈0.05）.The serum level of miRNA-101 was positively correlated with the glycosylated hemoglobin A1c（ HbA1c,P〈0.05）.Multiple linear regression analysis revealed that the circulating miRNA-101 was in significant positive correlation with HbA1c（ P〈0.05） after adjustment for age,sex and body weight.CONCLUSION： Enhanced circulating miRNA-101 level in newly diagnosed T2DM patients may be associated with elevation of HbA1c.