鱼糜酶解蛋白亚铁螯合肽微胶囊工艺及抗氧化研究

Preparation and Antioxidant Activity of Ferrous Chelating Peptide from Haitail Hydrolysis Microcapsules

以舟山带鱼鱼糜和FeCl_2为原料,采用水解-螯合法制备鱼糜亚铁螯合肽Fe(Ⅱ)-FPH。为了保护Fe(Ⅱ)-FPH不被空气氧化,选用双层包埋法,以大豆分离蛋白(SPI)和果胶为壁材制备微胶囊。通过单因素试验,选择最大包埋率的最佳工艺条件:壁芯比2∶1,壁材SPI与果胶质量之比45%∶55%,包埋时间30 min。对微胶囊产品的溶解度、吸水性、粒径及抗氧化效果等特性进行测定与评价。抗氧化试验结果显示:Fe(Ⅱ)-FPH微胶囊作为油脂的抗氧化性具有较强的抗氧化活性,其效果与维生素E相当;在试验前3 d,添加Fe(Ⅱ)-FPH微胶囊的大豆油POV值略高于只添加Fe(Ⅱ)-FPH的大豆油,然而3 d后明显低于只添加Fe(Ⅱ)-FPH的大豆油,说明Fe(Ⅱ)-FPH微胶囊有一定的缓释能力,壁材能抵御环境中氧分子对芯材螯合物的影响。

Zhoushan haitail surimi and FeCl_2 were used to preparehe haitail hydrolysis ferrous chelating peptide [Fe（Ⅱ）-FPH].In order to protect the Fe（Ⅱ）-FPH from oxygenated,SPI/pectin were used as the wall material for preparing the Fe（Ⅱ）-FPH microcapsules using the complex coacervation method.The result showed that the best encapsulation efficiency to make microcapsules is the wall material to core rate is 2∶1,SPI/pectin to 45%∶55% and the embedding time is 30 min,which has the perfect to the maximum embedding rate.The morphological characteristics,such as moisture,solubility,encapsulation efficiency and antioxidant activity were also tested and evaluated.The experimental results show the Fe（Ⅱ）-FPH microcapsules has strong antioxidant activity,the effect is similar to vitamin E.In the three days before the experiment,the POV value of soybean oil that added microcapsules is slightly higher than that only added Fe（Ⅱ）-FPH,but significantly lower than hat only added Fe（Ⅱ）-FPH3 days later.This phenomenon indicates that Fe（Ⅱ）-FPH microcapsules have some sustained ability and the wall material can protect Fe（Ⅱ）-FPH against environmentaloxidation.