栀子蓝色素的分步制备工艺

Production Technology of Gardenia Blue Pigment by Stepwise Method

优化栀子蓝色素分步制备的工艺。以栀子黄生产废液提取物——京尼平苷为底物原料,以黑曲霉发酵制备的β-葡萄糖苷酶为转色酶,研究京尼平苷水解制备京尼平的影响因素,通过正交设计试验优化其工艺条件。以显色生成亮丽栀子蓝色素为目标,筛选显色氨基酸,制备色泽明亮的栀子蓝色素。结果表明,在添加8%粗β-葡萄糖苷酶量,反应温度55℃,p H 4.2,水解时间7 h条件下,京尼平产量最高;甲硫氨酸、甘氨酸、赖氨酸、丙氨酸等多种氨基酸均与京尼平反应生成蓝色素,其中甲硫氨酸显色反应生成物色泽亮丽,是由栀子蓝色素制备的适宜氨基酸;n_(甲硫氨酸)∶n_(京尼平苷)=6∶1、p H 7.0、显色温度80℃、时间10 h时,获得色泽明亮的栀子蓝色素,其色价为20.6。

The production technology of gardenia blue pigment with stepwise method was studied. With the exhausted solution of gardenia yellow as the substrate material and β-glucosidase produced by Aspergillus niger fermentation as the enzyme for producing gardenia blue, the factors and production technology on hydrolysis of geniposide through orthogonal test was researched. With generating bright gardenia blue as the target, the suitable amino acids for coloration was also selected. Finally, the bright gardenia blue was obtained. The results showed that the optimum hydrolysis conditions of geniposide was as follows： the dosage of enzyme is 8%, temperature 55 ℃, p H 4.2, hydrolysis time 7 h. Methionine,glycine, lysine, alanine, etc. can be reacted with genipin to generate the blue pigment and the methionine is the optimal amino acid for the preparation of gardenia blue pigment. When p H 7.0, nMet∶ ngeniposidic= 6 ∶ 1, coloration temperature 80℃, coloration time 10 h, the bright gardenia blue pigment can be prepared out and the color value was 20.6.