肥胖相关蛋白FTO抑制人胰腺癌细胞增殖及机制的研究

Study on the function of fat mass and obesity-associated protein in pancreatic cancer cell

目的:研究肥胖相关蛋白FTO在胰腺癌细胞中的功能,及参与调控胰腺癌发生、发展的机制。方法:利用RT-q PCR和western blot来检测胰腺正常细胞和胰腺癌细胞中FTO的mRNA和蛋白的表达;利用MTT、CCK8、Brd U和TUNEL来检测胰腺癌细胞在siRNA干扰后的增殖和凋亡情况;利用斑点杂交等检测胰腺癌细胞mRNA中6-甲基腺苷的水平;结果:FTO在胰腺癌细胞中高表达;FTO调控胰腺癌细胞PANC-1的细胞增殖;FTO调节mRNA中6-甲基腺苷的水平;结论:FTO在胰腺癌中高表达,敲降胰腺癌细胞中的FTO会抑制细胞生长并增加凋亡,同时细胞DNA的合成减少以及甲基化mRNA的积累并调节mRNA稳定性,在胰腺癌细胞中起重要作用。

Objective： To investigate the function of obesity related protein FTO in pancreatic cancer cells and the possible regulatory mechanisms of pancreatic carcinogenesis and metastasis. Methods： The expression of FTO mRNA and protein in normal pancreatic cells and pancreatic cancer cells was detected with RT-q PCR and Western blot technologies as well as the proliferation and apoptosis of pancreatic cancer cells after siRNA interference with MTT,CCK8,Brd U and TUNEL technologies; the 6-methyl adenosine levels were detected in pancreatic cancer cells mRNA with dot hybridization. Results： FTO was found to be abundantly is highly expressed in pancreatic cancer cells; FTO regulated cell proliferation of PANC-1 cell and the level of 6-methyl adenosine in mRNA.Conclusion： FTO is highly expressed in pancreatic cancer,and knockdown of FTO in pancreatic cancer cells has inhibited cell growth and increased apoptosis,which plays an important role in pancreatic cancer cells; while the synthesis of DNA is reduced and mRNA methylation is accumulating to regulate mRNA stability.