嗜酸乳杆菌肽聚糖水解条件优化及抗炎活性研究

Optimization of Hydrolysis Conditions of Peptidoglycan from Lactobacillus acidophilus and Its Anti-inflammatory Activity

研究嗜酸乳杆菌肽聚糖的水解情况及产物抗炎活性,通过比较盐酸法和溶菌酶法,发现溶菌酶水解肽聚糖的效果显著。利用单因素法和响应面分析法,优化溶菌酶的水解时间,温度和E/S,得出水解肽聚糖的最佳工艺:温度38.2℃,时间3 h,E/S 1∶5。在对水解产物的细胞学研究中,采用MTT和WB试验,发现嗜酸乳杆菌肽聚糖(PGN)水解物对于LPS诱导的小鼠巨噬细胞RAW 264.7具有良好的抗炎活性。

This study revealed the relationship of peptidoglycan enzymatic hydrolysates and anti-inflammatory activity.Compared with hydrochloric acid method, we found that lysozyme had a significant effect in the hydrolysis process of peptidoglycan. With the methods of single factor and orthogonal analysis, hydrolysis time, temperature and E/S were screened as the three main factors affecting the production of peptidoglycan hydralysates. The optimized conditions were ：temperature 38.2 ℃, time 3 h, E/S 1∶5. Further research also found PGN hydralysates have potential anti-inflammatory activity in LPS-induced RAW 264.7 cells.