适于玉米基因图位克隆的SSR标记开发与应用

The Development and Application of SSR Markers Suitable for Map-Based Cloning in Maize

玉米(Zea mays)是重要的粮食作物和经济作物。自2009年玉米基因组测序完成以来,利用图位克隆技术对玉米基因的克隆与功能分析成为研究热点。从ES1定位的基因组区间共搜索出59 144个SSR位点,对其中部分位点比对分析,找到1 304个单拷贝的SSR位点。我们合成了所有单拷贝SSR位点的引物,利用PAGE电泳检测筛选出在两个亲本之间具有多态性的SSR位点182条,检出率为14.0%。BSA法检验多态性SSR位点,筛选出与ES1紧密连锁的SSR分子标记63个,检出率为34.6%,用于基因克隆。该方法可简便、快捷的筛选到与目的基因连锁的SSR标记,有助于快速克隆到目的基因。

Maize（Zea mays） is an important food and economic crops. Mapping-based cloning and functional analysis of maize genes have been the worldwide research highlights since the maize genome was sequenced in2009. In the fine mapping section of ES1 in the whole genome, we sought out 59 144 SSR sites, found out 1 304 sites which were single copes through blast. The primers of all single cope sites were synthesized. 182 polymorphism SSR markers between two parents were selected out by PAGE electrophoresis and the proportion was 14.0%.Utilize BSA, found out 63 SSR markers linked with ES1 and the proportion was 34.6%. These markers can be used for the fine mapping. This method can simply and conveniently screen out the SSR markers which link the target gene, as well as contribute to quickly find out the target gene.