张家界部分杂柚品种基于SRAP分子标记的亲缘关系分析

Genetic Relationship Analysis of Part Hybrid Pummelo in Zhangjiajie Based on SRAP Molecular Markers

采用SRAP分子标记技术对包含张家界地区4个杂柚品种在内的9个柑橘品种进行遗传多样性及亲缘关系分析。结果表明:45对引物共扩增得到467个条带,其中多态性条带354个,多态性百分率为75.8%,因此SRAP可以作为柚类资源亲缘关系研究的有效标记。在遗传相似系数0.623 1处,供试材料可区分为两大组,第一组为沙田柚、慈利香柚、菊花芯柚、慈利金香柚,第二组包括慈利菠萝香柚、大分4号、慈利水柚子、大红甜橙、兴山酸橙。聚类结果表明4个柚类杂种中,慈利香柚和慈利金香柚与沙田柚及菊花芯柚的亲缘关系较近,而与大红甜橙、兴山酸橙及大分4号的亲缘关系则相距较远;而慈利菠萝香柚和慈利水柚子则刚好相反,它们更偏向于大红甜橙、兴山酸橙及大分4号,而与张家界主栽的两个柚类品种的亲缘关系则较远。

Genetic relationship and genetic diversity of 9 citrus varieties were studied based on sequence-related amplified polymorphism（SRAP） molecular markers. The main results were as follows： A total of 467 bands were obtained with 45 SRAP primers, and 354 bands out of them were polymorphic. The diversity ratio was 75.80%.The results showed that SRAP could be as an efficient technique to assess genetic diversity for pummelo. The dendrogram showed that all varieties could be divided into two clusters at the similarity level of 0.6231,Shatianyou, Cilixiangyou, Juhuaxinyou and Cilijinxiangyou into the first group; Ciliboluoxiangyou, Dafen 4,Cilishuiyouzi, Dahong Sweet Orange and Xinshan Sour Orange into the second group. The relationship between Cilixiangyou and Shatianyou, Cilijinxiangyou and Juhuaxinyou, Ciliboluoxiangyou and Dahong Sweet Orange,Cilishuiyouzi and Dahong Sweet Orange is close, whereas Cilixiangyou and Dafen 4, Cilijinxiangyou and Xinshan Sour Orange, Ciliboluoxiangyou and Shatianyou, Cilishuiyouzi and Shatianyou is distant.